M4071
MINK1 (1-320), active, GST tagged human
PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym(s):
B55, Hmink, MAP4K6, MGC21111, YSK2, ZC3, hMINKbeta
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.32
Recommended Products
recombinant
expressed in baculovirus infected Sf9 cells
Quality Level
product line
PRECISIO® Kinase
Assay
≥70% (SDS-PAGE)
form
buffered aqueous glycerol solution
specific activity
16-22 nmol/min·mg
mol wt
~61 kDa
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... MINK1(50488)
General description
Misshapen/NIKs-related kinase 1 (MINK1), also known as hMNIKβ or MINK is mapped to human chromosome 17p13.1. The gene codes for serine/threonine kinase, which is a member of the germinal center family of kinases (GCK) that are homologous to the yeast sterile 20 (Ste20) kinase family.
Biochem/physiol Actions
Misshapen/NIKs-related kinase 1 (MINK1) plays a vital role in modulating various cellular process such as actin cytoskeleton rearrangement, cell matrix adhesion, cell-cell adhesion, cell morphology, and survival. It is also implicated in the cell motility and cell invasion. Kinase inactive mutant of MINK1 increased cell spreading, actin stress fiber formation, adhesion to extracellular matrix and inhibited cell motility and invasion. Thus, alteration in the MINK1 activity inhibits tumor development. MINK is activated after Ras induction via a method involving reactive oxygen species (ROS). This activated MINK triggers the activity of p38 stress-activated kinase via MAP3K (Mitogen-Activated Protein Kinase Kinase Kinase) 5/8 and MKK (mitogen-activated protein kinase kinase) 3/6, which leads to p53-independent induction of cyclin-dependent kinase inhibitor, p21WAF1/CIP1 and permanent cell cycle arrest. MINK1 has an essential role in abscission, which is a final stage of cytokinesis.
Physical form
Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.2 5mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.
Legal Information
PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Misshapen-like kinase 1 (MINK1) is a novel component of striatin-interacting phosphatase and kinase (STRIPAK) and is required for the completion of cytokinesis.
Hyodo T
The Journal of Biological Chemistry, 287(30), 25019-25029 (2012)
Involvement of MINK, a Ste20 family kinase, in Ras oncogene-induced growth arrest in human ovarian surface epithelial cells.
Nicke B
Molecular Cell, 20(5), 673-685 (2005)
H Ozcelik
Cloning and Characterization of Expanded CAG-Repeat Containing Sequence(s): Identification of Candidate Breast Cancer Gene(s). (2003)
Identification and functional characterization of a novel human misshapen/Nck interacting kinase-related kinase, hMINK beta.
Hu Y
The Journal of Biological Chemistry, 279(52), 54387-54397 (2004)
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