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M0643

Sigma-Aldrich

Minimum Essential Medium Eagle

With Earle′s salts, L-glutamine, and non-essential amino acids, without sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

EMEM, MEM

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

Quality Level

500

form

powder

technique(s)

cell culture | mammalian: suitable

components

HEPES: no
Earle’s salts (5% CO2): yes
NaHCO3: no
phenol red: yes
L-glutamine: yes
sodium pyruvate: no

shipped in

ambient

storage temp.

2-8°C

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General description

It contains 19 amino acids. The essential amino acids and the non-essential amino acids; L-ala; L-asn; L-asp; L-glu; L-gly; L-pro and L-ser. May be preferred for growth of adherent cells in the presence of reduced serum or as a base for development of a serum free medium.
Minimum Essential Medium Eagle consists of inorganic salts, water-soluble vitamins, sugar, and essential amino acids. It is widely used as a media formulation for many cell culture studies. It is a modified version of Basal medium Eagle (BME) and the concentrations of the majority of amino acids are twice as compared to BME.

Application

Minimum Essential Medium Eagle has been used to culture osteoblast-like cell line MG-63, dispersed ventricular cells, and endometrial adenocarcinoma Ishikawa cells.

Quantity

Formulated to contain 9.6 grams of powder per liter of medium.

Reconstitution

Supplement with 2.2 g/L sodium bicarbonate.

also commonly purchased with this product

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supplement

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Description
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Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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János Magyar et al.
European journal of pharmacology, 487(1-3), 29-36 (2004-03-23)
Concentration-dependent (10-1000 microM) effects of terpenoid phenol derivatives were studied on L-type Ca(2+) current in isolated canine and human ventricular cardiomyocytes using the whole-cell configuration of patch clamp technique. Carvacrol, thymol and eugenol suppressed peak Ca(2+) current at +5 mV
Atomic layer deposited TiO2 films on an equiatomic NiTi shape memory alloy for biomedical applications
Abbas A, et al.
Journal of alloys and compounds, 161282-161282 (2021)
Tatsuma Yao et al.
Reproductive medicine and biology, 16(2), 99-117 (2017-12-21)
Cell culture technology has spread prolifically within a century, a variety of culture media has been designed. This review goes through the history, characteristics and current issues of animal-cell culture media. A literature search was performed on PubMed and Google
Sarah M Davis et al.
Molecular therapy. Nucleic acids, 21, 266-277 (2020-07-02)
Small interfering RNAs (siRNAs) have the potential to treat a broad range of diseases. siRNAs need to be extensively chemically modified to improve their bioavailability, safety, and stability in vivo. However, chemical modifications variably impact target silencing for different siRNA sequences
Hongjie Fan et al.
Cells, 10(11) (2021-11-28)
Bisphenol A (BPA) is a well-known endocrine disruptor, widely used in various consumer products and ubiquitously found in air, water, food, dust, and sewage leachates. Recently, several countries have restricted the use of BPA and replaced them with bisphenol S
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Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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