GE80-6484-51

2-D Clean-Up Kit

Cytiva 80-6484-51

• Synonym(s): 2D Clean-Up Kit
• UNSPSC Code: 41105323
• NACRES: NA.25

Properties

• feature: wetted part: no
• manufacturer/tradename: Cytiva 80-6484-51
• packaging: pkg of 50 samples

Description

General description

2-D Clean-Up Kit is designed to prepare samples for 2-D electrophoresis that otherwise produce poor 2-D spot-maps due to high conductivity, high levels of interfering substances, or low protein concentration. The reagents quantitatively precipitate proteins while leaving interfering substances, such as detergents, salts, lipids, phenolics, and nucleic acids, in solution.

Treatment of the sample with 2-D Clean-Up Kit can improve the quality of 2-D electrophoresis results, reducing streaking, background staining, and other artifacts due to interfering contaminants. The kit can make 2-D analysis possible for samples that are otherwise too dirty or too dilute.

The 2-D Clean-Up Kit procedure uses precipitant and co-precipitant in combination to quantitatively precipitate proteins. Precipitated proteins are pelleted by centrifugation and the precipitate is further washed to remove nonprotein contaminants. After a second centrifugation, the resultant pellet is resuspended into denaturing sample solution for first-dimension IEF.

The procedure can be completed in 90 min and does not result in spot gain or loss. The kit contains sufficient reagents to process 50 samples of up to 100 μ each. The procedure can be scaled up for larger volumes or more dilute samples.

2-D Clean-Up Kit increases labeling efficiency and protein abundance when using 2-D Fluorescence Difference Gel Electrophoresis (2-D DIGE).

Application

Automated identification and quantitation of proteins and peptides using LC-MS/MS.

Features and Benefits

• Improves the quality of 2-D electrophoresis results by removing interfering contaminants
• Quantitative precipitation separates proteins from detergents, salts, lipids, phenolics and nucleic acids
• Delivers quantitative yield in about 90 min.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

The wash buffer should be placed at -20 °C at least 1 h before use and may be s-red in a -20 °C freezer

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Safety Information

• Pictograms: GHS09
• Signal Word: Danger
• Hazard Statements: H314,H335,H411
• Precautionary Statements: P280 - P273 - P304 + P340 + P310 - P301 + P310 + P331 - P303 + P361 + P353 + P310 - P305 - P310 - P405 - P501
• Storage Class Code: 8B - Non-combustible corrosive hazardous materials