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F7879

Sigma-Aldrich

Ferritin, cationized from horse spleen

10 mg/mL in 0.15 M NaCl

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.26

biological source

horse spleen

Quality Level

sterility

sterile-filtered

form

liquid

concentration

10 mg/mL in 0.15 M NaCl

technique(s)

cell culture | mammalian: suitable
electron microscopy: suitable

UniProt accession no.

storage temp.

2-8°C

Gene Information

horse ... FTL(100051593)

General description

Ferritin, an iron-storage protein is usually present in the liver and spleen in mammals. Iron and ferritin are distributed relatively same in the eye. Ferritin is made up of heavy and light chains.

Application

A cationic derivative used to label negatively charged cell membranes for visualization by electron microscopy.
Ferritin is an iron storage protein that plays a central role in iron metabolism, and may be elevated in inflammatory or malignant diseases. It has been used in studies as an indicator of body iron supply, but may prove inaccurate in in the presence of chronic inflammation.
Ferritin, cationized from horse spleen has been used:
  • to incubate amoebae (106) in the study, to determine whether trophozoites are able to take up ferritin and internalise this protein for their growth in axenic culture
  • to determine phagosome-lysosome fusion by electron microscopy
  • to label freshly excised chick optic tecta in serum free incubation medium

Biochem/physiol Actions

Ferritin holds iron at its ferric state (Fe3+). The core of ferritin has the capability to hold 4500 iron molecules. It is essential to store iron in vertebrates. Ferritin is also essential to store and transport iron in invertebrates. It plays a key role in dietary iron absorption.

Preparation Note

Prepared by coupling horse spleen ferritin with N,N-dimethyl-1,3-propanediamine (DMPA).

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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R H Adamson et al.
The Journal of physiology, 445, 473-486 (1992-01-01)
1. We have investigated the interaction of plasma proteins with the endothelial cell using cationized ferritin as a marker of the cell surface glycocalyx. 2. Single microvessels of the frog mesentery were sequentially perfused using glass micropipettes with solutions containing
Interaction of Legionella pneumophila with Acanthamoeba castellanii: uptake by coiling phagocytosis and inhibition of phagosome-lysosome fusion.
Bozue J A and Johnson W
Infection and Immunity, 64(2), 668-673 (1996)
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Once penetrated into the lungs of exposed people, asbestos induces an in vivo biomineralisation process that leads to the formation of a ferruginous coating embedding the fibres. The ensemble of the fibre and the coating is referred to as asbestos
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A significant barrier to biomarker development in the field of acute kidney injury (AKI) is the use of kidney function to identify candidates. Progress in imaging technology makes it possible to detect early structural changes prior to a decline in

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