F6928
Monoclonal Anti-FKHR (FOXO1a) antibody produced in mouse
clone FKH 117, purified immunoglobulin, buffered aqueous solution
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About This Item
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biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
FKH 117, monoclonal
form
buffered aqueous solution
mol wt
antigen 70-75 kDa
species reactivity
human
technique(s)
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.5-1 μg/mL using total extract of COS-7 cells transfected with FKHR expression plasmid
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... FOXO1(2308)
General description
FKHR belongs to the superfamily of forkhead transcription factors (FOX). It is characterized by a common Forkhead (or winged helix) domain, a variant of the helix-turn-helix motif. FKHR contains three residues, Thr24, Ser256, and Ser319 that lie within consensus sequences for phosphorylation by protein kinase B (PKB).
Monoclonal Anti-FKHR (FOXO1a) (mouse IgG1 isotype) is derived from the FKH117 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to human FKHR, conjugated to KLH.
Immunogen
synthetic peptide corresponding to human FKHR (amino acids 636-651), conjugated to KLH.
Application
Monoclonal Anti-FKHR (FOXO1a) antibody is suitable for immunoblot assay to study the down-regulation of both p16INK4A and p14ARF expression, and immunoblot of SKMCs and HSMM cells in a study of stable ectopic expression of PAX3-FKHR in low passage primary human skeletal muscle cells. It is also suitable for microarray and indirect ELISA.
Monoclonal Anti-FKHR antibody has been used in:
- immunoblotting
- enzyme linked immunosorbent assay (ELISA)
- immunoprecipitation
Biochem/physiol Actions
FKHR plays a key regulatory roles in embryonic development, differentiation, apoptosis, and tumorigenesis. It was first identified at chromosomal breakpoints in human tumors and consequently linked to tumorigenesis. The activity of FKHR is regulated by growth factors via the PKB/PI3K pathway. The expression of FKHR is inhibited through phosphorylation by PKB. Within the nucleus, the dephosphorylated FKHRL1 induces expression of target genes such as Fas ligand, and triggers apoptosis. It is involved in apoptosis, glucose homeostasis, cell cycle regulation and as a nuclear receptor co factor. It has been found that FKHR also play a role in endometrial differentiation via the protein kinase A (PKA) pathway.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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A Mansouri
Critical reviews in oncogenesis, 9(2), 141-149 (1999-02-11)
Pax genes consist of a family of transcription factors that are essentially required for the genesis of a variety of tissues and organs. Pax3 and Pax7 are specifically expressed in the dorsal neural tube and the developing somite. Loss-of-function mutations
The PAX3-FKHR Fusion Gene of Rhabdomyosarcoma Cooperates with Loss of p16INK4A to Promote Bypass of Cellular Senescence
Linardic CM, et al.
Cancer Research, 67(14), 6691-6699 (2007)
FGFR4 Blockade Exerts Distinct Antitumorigenic Effects in Human Embryonal versus Alveolar Rhabdomyosarcoma
Crose LES, et al.
Clinical Cancer Research, 18(14), 3780-3790 (2012)
E D Tang et al.
The Journal of biological chemistry, 274(24), 16741-16746 (1999-06-08)
The FKHR gene was first identified from its disruption by the t(2;13) chromosomal translocation seen in the pediatric tumor alveolar rhabdomyosarcoma. It encodes for a member of the forkhead family of transcription factors. Recently, a homolog of FKHR in the
J Hillion et al.
Blood, 90(9), 3714-3719 (1997-11-14)
Fusion genes implicating the MLL gene have been recently demonstrated in various 11q23 chromosomal abnormalities in human hematopoietic malignancies. We analyzed a t(6;11)(q21;q23) translocation detected in a secondary acute myeloblastic leukemia. This translocation results in fusion of the MLL gene
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