Skip to Content
Merck
All Photos(1)

Documents

D3160

Sigma-Aldrich

Deoxyribonucleic acid from human placenta

buffered aqueous solution, sexed, male

Synonym(s):

DNA

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

CAS Number:
9007-49-2
MDL number:
MFCD00130921
UNSPSC Code:
12352200

grade

for molecular biology

form

buffered aqueous solution

shipped in

dry ice

storage temp.

−20°C

InChI

1S/C15H31N3O13P2/c16-13-1-7(20)11(28-13)5-25-32(21,22)31-9-3-15(18)29-12(9)6-26-33(23,24)30-8-2-14(17)27-10(8)4-19/h7-15,19-20H,1-6,16-18H2,(H,21,22)(H,23,24)

InChI key

AWBASQCACWFTGD-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

General description

Human placental DNA is isolated from donor placenta, but will contain some maternal DNA. The DNA fragments are sonicated to produce fragments of consistent size.

Application

Deoxyribonucleic acid from human placenta is used as blocking agent in Southern hybridizations. It was used to compare the efficiency of DNA quantification methods.
For use in Southern hybridizations.

Components

DNA is supplied in a solution of 10mM Tris-HCl (pH 8.4), with 1mM EDTA.

related product

Product No.
Description
Pricing

D4522

Deoxyribonucleic acid from calf thymus, Type XV, Activated, lyophilized powder

D3287

Deoxyribonucleic acid, single stranded from human placenta

D4889

Deoxyribonucleic acid sodium salt from Escherichia coli strain B, Genomic, unsheared

D7290

Deoxyribonucleic acid from herring sperm, single-stranded for hybridization

D7656

Deoxyribonucleic acid, single stranded from salmon testes, For hybridization

D9156

Deoxyribonucleic acid, single stranded from salmon testes, For hybridization

D3035

Deoxyribonucleic acid from human placenta, buffered aqueous solution, sexed, female

D8661

Deoxyribonucleic acid solution from calf thymus, For hybridization

S8140

SSPE Buffer, for Northern and Southern blotting, powder blend

N6019

Neutralizing Buffer, for Northern and Southern blotting, powder

N1531

Denaturation Solution, for Northern and Southern blotting

B6429

Casein Blocking Buffer 10x, for Northern and Southern blotting, solution

S2015

SSPE Buffer 20× Concentrate, for Northern and Southern blotting, solution

M5755

MOPS-EDTA-Sodium Acetate (MESA), powder for RNA electrophoresis buffers

S0902

SSC Buffer, for Northern and Southern blotting, powder blend

H7033

PerfectHyb™ Plus Hybridization Buffer, for Northern and Southern blotting, solution

S8015

SSC Buffer, for Northern and Southern blotting, powder blend

S6639

SSC Buffer 20× Concentrate, for Northern and Southern blotting, solution

P1415

Prehybridization Solution, for Northern and Southern blotting, liquid (2x concentrate)

C7594

Casein Blocking Buffer, for Northern and Southern blotting, powder blend

15356

Blotting-Nylon 66 membranes, type B, positive, for Northern and Southern blotting

31149

Deoxyribonucleic acid, low molecular weight from salmon sperm

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Karsten Nielsen et al.
Forensic science international. Genetics, 2(3), 226-230 (2008-12-17)
Six commercial preparations of human genomic DNA were quantified using five quantification methods: UV spectrometry, SYBR-Green dye staining, slot blot hybridization with the probe D17Z1, Quantifiler Human DNA Quantification kit and RB1 rt-PCR. All methods measured higher DNA concentrations than
Zachary D Smith et al.
Nature, 511(7511), 611-615 (2014-08-01)
In mammals, cytosine methylation is predominantly restricted to CpG dinucleotides and stably distributed across the genome, with local, cell-type-specific regulation directed by DNA binding factors. This comparatively static landscape is in marked contrast with the events of fertilization, during which
Manolis Kellis et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(17), 6131-6138 (2014-04-23)
With the completion of the human genome sequence, attention turned to identifying and annotating its functional DNA elements. As a complement to genetic and comparative genomics approaches, the Encyclopedia of DNA Elements Project was launched to contribute maps of RNA
Maarten Holkers et al.
Nature methods, 11(10), 1051-1057 (2014-08-26)
Engineered sequence-specific nucleases and donor DNA templates can be customized to edit mammalian genomes via the homologous recombination (HR) pathway. Here we report that the nature of the donor DNA greatly affects the specificity and accuracy of the editing process
Emilia Huerta-Sánchez et al.
Nature, 512(7513), 194-197 (2014-07-22)
As modern humans migrated out of Africa, they encountered many new environmental conditions, including greater temperature extremes, different pathogens and higher altitudes. These diverse environments are likely to have acted as agents of natural selection and to have led to
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service