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WI 38 Cell Line human

90020107, human lung (fetal), Fibroblast-like

Synonym(s):

WI38 Cells, Wistar Institute-38 Cells

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About This Item

UNSPSC Code:
41106514

product name

WI 38 Cell Line human, caucasian fibroblast-like fetal lung cell, 90020107

biological source

human lung (fetal)

growth mode

Adherent

karyotype

2n = 46, diploid except at high passage number

morphology

Fibroblast-like

products

Collagen

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Human Caucasian foetal lung

Cell Line Description

From normal embryonic lung of female. Have very broad human virus spectra, especially useful for isolation of rhinoviruses. Cells form multilayered membrane when held for long periods at 37°C with periodic pH adjustments. Used in preparation of human virus vaccines.

Application

From normal female embryonic lung (expect collagen production). These cells have a very broad human virus spectra. Particularly useful for the isolation of rhinovirus. Cells from multilatered membrane when incubated for long periods with periodic pH adjustments. Used in the preparation of human virus vaccines.
Subculture: Spit at 70-80% confluency, approx. 1:3 to 1:6, seed at 2-4x104 viable cells/cm2. Trypsinize using 0.25% solution, with or without EDTA, 37 °C and 5% CO2.
Virus studies (rhinoviruses), vaccine production
WI 38 human cell line has also been used to study the cytotoxicity of acrylic based resin compounds and silica nanoparticles.

Biochem/physiol Actions

Finite lifespan. Approx. 50, +/- 10, population doublings. Normal doubling time is 24 hours.

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 10,12
D13S317: 11
D16S539: 11,12
D5S818: 10
D7S820: 9,11
THO1: 8,9.3
TPOX: 8
vWA: 19,20

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Finite lifespan, 50 plus or minus 10 population doublings expected - doubling time 24h.

Analysis Note

Recommended culture medium: EMEM (EBSS) + 2 mM L-Glutamine + 1% Non-essential amino acids (NEAA) + 15% FBS

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

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