B6312

14-3-3 Beta histidine-tagged human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE), buffered aqueous glycerol solution

• Synonym(s): 14-3-3 β, 14-3-3 protein Beta, GW128, HS1, KCIP-1 protein 1054, YWHAB
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• recombinant: expressed in E. coli
• Quality Level: 200
• Assay: ≥90% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• UniProt accession no.: P31946
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... YWHAB(7529)

Description

Biochem/physiol Actions

14-3-3 proteins are a multifunctional protein family comprising seven mammalian isoforms that interact with over two hundred different intracellular molecules including kinases, phosphatases, transcription factors, scaffold proteins, and DNA. 14-3-3 proteins are involved in a multitude of biological functions and play a regulatory role in processes such as apoptotic cell death, mitogenic signal transduction, and cell cycle control. 14-3-3 β participates in various processes, among which is the positive regulation of cell cycle progression and tumorigenesis. Over-expression of 14-3-3 β in NIH-3T3 cells confers tumorigenicity in nude mice via activation of the mitogen-activated protein kinase (MAPK) cascade.

Physical form

Supplied as a 0.2 μm filtered solution in 50 mM Tris, pH 7.6, 1 mM EDTA, 1 mM DTT and 20% glycerol (w/v).

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves