B3679
BCIP®/NBT-Blue
alkaline phosphatase substrate, chromogenic, liquid
Synonym(s):
5-bromo-4-chloro-3-indolyl-phosphate / Nitro blue tetrazolium chloride liquid substrate system
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
| Size/SKU | Availability | Price |
|---|
About This Item
UNSPSC Code:
12352204
NACRES:
NA.83
Pricing and availability is not currently available.
Product Name
BCIP®/NBT-Purple Liquid Substrate System for Membranes, ready to use alkaline phosphatase substrate
Quality Segment
form
liquid
technique(s)
protein detection: suitable
storage temp.
2-8°C
Application
BCIP®/NBT-Purple Liquid Substrate System for Membranes has been used as a substrate for alkaline phosphatase
- for the detection of secondary antibodies by immunoblot
- in ELISPOT assay
- in dot blot analysis
- in western blot to visualize protein bands on nitrocellulose membrane
Biochem/physiol Actions
5-bromo-4-chloro-3-indolyl phosphate (BCIP)/ nitro blue tetrazolium (NBT) is a chromogenic substrate system for the detection of alkaline phosphatase (AP) enzyme. AP hydrolyses BCIP to form an intermediate leucoindigo compound and is further oxidized by NBT to form 5,5′-dibromo-4,4′-dichloro indigo (BCI). NBT is reduced to an insoluble purple diformazan (DF) and forms NBT-DF. A mixture of NBT-DF and BCI results in the dark purple colored precipitate. AP by hydrolysing many BCIP molecules makes it a highly sensitive method.
Physical form
Ready-to-use.
Legal Information
BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Stable at least one year at 2-8 °C.
1 of 1
This Item | |||
|---|---|---|---|
| description ready to use alkaline phosphatase substrate | description alkaline phosphatase substrate | description ready to use solution | description - |
| Quality Level 100 | Quality Level 100 | Quality Level 300 | Quality Level 100 |
| form liquid | form liquid | form liquid | form liquid |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. - |
Still not finding the right product?
Explore all of our products under BCIP®/NBT-Purple Liquid Substrate System for Membranes
Signal Word
Warning
Hazard Codes
Precautionary Statements
Hazard Classifications
Skin Sens. 1
Storage Class
10 - Combustible liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.