Achromopeptidase from bacteria

Name: Achromopeptidase from bacteria
Brand: SIGMA

partially purified powder, ≥20,000 units/mg solid

Synonym(s):

ACP, Achromopeptidase

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About This Item

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

3.4.21.50 (BRENDA, IUBMB)

MDL number:

MFCD00130333

Specific activity:

≥20,000 units/mg solid

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Properties

form

partially purified powder

Quality Segment

200

specific activity

≥20,000 units/mg solid

mol wt

27 kDa

foreign activity

Collagenase, present

storage temp.

−20°C

Description

Application

Achromopeptidase is useful for lysis of Gram-positive bacteria that are resistant to lysozyme. Achromopeptidase, from Sigma, has been used for the isolation and molecular characterization of skin and soft-tissue bacterial infections. Product A3422 is from bacteria and is a partially purified powder containing lactose.

Achromopeptidase from bacteria may be used:

in the homogenization step for genomic DNA extraction from microbial biofilm
in the lysis of gram-positive strains(
for DNA extraction from Nocardia strains

Biochem/physiol Actions

Achromopeptidase is a lysyl endopeptidase that has a molecular weight of approximately 27 kDa. It works best between pH 8.5 -9. Approximately 500-1,500 μn/ml achromopetidase can be used to lyse cells at a density of OD₆₀₀= 0.6 over 2 hours at 37 °C.

Achromopeptidase is a bacteriolytic enzyme and belongs to serine protease family. It is effective for the lysis of gram positive bacteria compared to lysozyme. Achromopeptidase activity is inhibited in the presence of heavy metals. Achromopeptidase based inhibition assay may be useful for screening the presence of heavy metals.

Physical form

Partially purified powder.

Other Notes

One unit will lyse 0.4 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 8.0 at 37 °C.

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This Item	G0751	A7646	A3903
Sigma-Aldrich A3422 Achromopeptidase from bacteria	Sigma-Aldrich G0751 β-Glucuronidase from Helix pomatia	Sigma-Aldrich A7646 Apyrase from potatoes	Sigma-Aldrich A3903 1-Aminocyclopropanecarboxylic acid
description partially purified powder, ≥20,000 units/mg solid	description Type H-1, partially purified powder, ≥300,000 units/g solid	description ATPase ≥60 units/mg protein, lyophilized powder (partially purified)	description ≥98% (TLC), powder
specific activity ≥20,000 units/mg solid	specific activity ≥300,000 units/g solid	specific activity -	specific activity -
form partially purified powder	form partially purified powder	form lyophilized powder (partially purified)	form powder
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. -
mol wt 27 kDa	mol wt -	mol wt ~49 kDa by gel filtration	mol wt -
foreign activity Collagenase, present	foreign activity -	foreign activity Acid Phosphatase ≤5% of base activity	foreign activity -
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200