A1908
Asialofetuin from fetal calf serum
Type II (Sigma designation)
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About This Item
Form:
powder
Biological source:
bovine
biological source
bovine
Quality Level
type
Type II (Sigma designation)
form
powder
technique(s)
activity assay: suitable, inhibition assay: suitable
impurities
salt, essentially free, ≤1.0% N-acetylneuraminic acid
solubility
H2O: soluble 1.0 mg/mL, clear to hazy, colorless to very faintly yellow
storage temp.
2-8°C
Application
Asialofetuin from fetal calf serum is suitable for use:
- in the assay of the lactose binding activity of the galectin-7 by immunodetection of in situ binding activity to asialofetuin immobilized on nitrocellulose
- in the inhibition of zona pellucida hardening of mouse oocytes
- as an acceptor in sialyltransferase enzyme activity assay
Incorporation of asialofetuin (AF) in liposomes strongly enhance delivery to and endocytosis by cells displaying the AF receptor, notably hepatocytes. This provides a very efficient route for gene therapy.
Biochem/physiol Actions
Feutins are glycoproteins that belong to the cystatin superfamily. They are characterized by the presence of two tandemly arranged cystatin-like domains and an unrelated domain. It binds to transforming growth factor (TGF)-β/BMP (bone morphogenic protein) cytokines.
Asialofetuin (AF) binds to asialoglycoprotein receptor (ASGP-R) and is employed as a ligand for drug delivery to the hepatocytes. It aids serum-resistant lipoplexes for efficient gene deliver into the HepG2 (human hepatoblastoma) cells.
Preparation Note
Enzymatic cleavage using neuraminidase
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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W M Brown et al.
BioEssays : news and reviews in molecular, cellular and developmental biology, 14(11), 749-755 (1992-11-01)
Bovine fetuin, the first fetal protein to be described, has recently been shown to be a species homologue of a well known human plasma protein--alpha 2HS glycoprotein (alpha 2HS). The fetuins are now known to be members of the cystatin
Serum-resistant lipoplexes in the presence of asialofetuin
Liposomes, 2011, 425-434 (2010)
X C Wang et al.
The Journal of biological chemistry, 264(3), 1854-1859 (1989-01-25)
The hepatic acute phase response is accompanied by increased levels of Gal beta 1-4GlcNAc alpha 2,6-sialyltransferase activity in liver and in circulation. Previous studies suggested that cytokines and glucocorticoids mediate the induction of this sialyltransferase activity. In this study the
Global Trade Item Number
| SKU | GTIN |
|---|---|
| A1908-50MG | 04061833257890 |
| A1908-10MG | 04061831151886 |
| A1908-5MG | 04061831151893 |