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88596

Sigma-Aldrich

Fluorescein-O′-acetic acid

BioReagent, suitable for fluorescence, ≥90% (HPCE)

Synonym(s):

FOAA, O′-(Carboxymethyl)fluorescein

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About This Item

Empirical Formula (Hill Notation):
C22H14O7
CAS Number:
233759-98-3
Molecular Weight:
390.34
MDL number:
MFCD05865263
UNSPSC Code:
12352106
PubChem Substance ID:
57653272
NACRES:
NA.32

product line

BioReagent

Quality Level

100

Assay

≥90% (HPCE)

form

powder

fluorescence

λex 455 nm; λem 514 nm in 0.1 M Tris pH 8.0

suitability

suitable for fluorescence

SMILES string

OC(=O)COc1ccc2c(OC3=CC(=O)C=CC3=C2c4ccccc4C(O)=O)c1

InChI

1S/C22H14O7/c23-12-5-7-16-18(9-12)29-19-10-13(28-11-20(24)25)6-8-17(19)21(16)14-3-1-2-4-15(14)22(26)27/h1-10H,11H2,(H,24,25)(H,26,27)

InChI key

VQKDLLIKXLDPHW-UHFFFAOYSA-N

Other Notes

Fluorescent label which reacts with amines after activation to give isomerically homogeneous derivatives

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H315,H319

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Analytica Chimica Acta, 423, 77-77 (2000)
H Wang et al.
Analytical biochemistry, 281(1), 15-20 (2000-06-10)
A new amine-reactive derivatizing reagent, N-hydroxysuccinimidyl fluorescein-O-acetate (SIFA), was developed for catecholamine (CA) analysis in liquid chromatography. The reactivity of this reagent with the CAs norepinephrine (NE), epinephrine (E), and dopamine (DA) was investigated in detail. In aqueous methanol containing

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