M8802

Malonate broth

pH 6.7±0.2 (25 °C), NutriSelect^® Plus, suitable for microbiology

• UNSPSC Code: 41171607
• NACRES: NA.85

Properties

• sterility: non-sterile
• Quality Level: 200
• form: powder
• manufacturer/tradename: NutriSelect^® Plus
• technique(s): microbe id | utilization test: suitable; microbiological culture: suitable
• pH: 6.7±0.2 (25 °C)
• application(s): food and beverages; water monitoring; microbiology
• suitability: Klebsiella spp.; Salmonella spp.; nonselective and differential for Enterobacter spp.; nonselective and differential for Escherichia coli; nonselective and differential for coliforms

Description

General description

Malonate broth is recommended for the differentiation of Enterobacter and Escherichia.

Application

Malonate broth has been used to evaluate the utilization of malonate by Enterobacter sakazakii. It has also been used to differentiate between Cronobacter sakazakii and Cronobacter malonaticus isolates.

Components

Components (g/L)
Ammonium sulfate, 2.00
Dipotassium phosphate, 0.60
Monopotassium phosphate, 0.40
Sodium chloride, 2.00
Sodium malonate, 3.00
Bromo thymol blue, 0.025

Preparation Note

Suspend 8 g of Malonate Broth in 1000 ml of distilled water. Dispense and sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Avoid the addition of carbon and nitrogen from other sources.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves