M1678

MUG EC broth

NutriSelect^® Plus, powder, selective and differential for Escherichia coli, pack of 500 g, suitable for microbiology

• Synonym(s): 4-Methylumbelliferyl β-D-Glucuronide Escherichia coli Broth
• NACRES: NA.85
• UNSPSC Code: 41171606
• Manufacturer/tradename: NutriSelect^® Plus
• Form: powder
• Suitability: selective and differential for Escherichia coli, selective and differential for coliforms
• Application(s): agriculture; environmental; food and beverages; veterinary; water monitoring, microbiology

Properties

• sterility: non-sterile
• Quality Segment: 200
• form: powder
• shelf life: limited shelf life, expiry date on the label
• packaging: pack of 500 g
• manufacturer/tradename: NutriSelect^® Plus
• technique(s): microbiological culture: suitable
• color: light brown
• final pH: 6.9±0.2 (25 °C)
• application(s): agriculture; environmental; food and beverages; veterinary; water monitoring, microbiology
• suitability: selective and differential for Escherichia coli, selective and differential for coliforms

Description

General description

MUG EC broth is a selective and differential liquid culture mediumspecifically formulated for the rapid detection of Escherichia coli inmicrobiological samples. The medium is based on EC broth components—caseinenzymatic hydrolysate as the source of essential nutrients, lactose as afermentable carbohydrate, bile salts, buffer salts, and sodium chloride tomaintain osmotic equilibrium—and is supplemented with the fluorogenic substrate4‑methylumbelliferyl β‑D‑glucuronide (MUG). MUGis hydrolyzed by the E. coli enzyme β‑glucuronidase to yield the fluorescent product 4‑methylumbelliferone, which can be observed under long‑wave UV light, enabling rapid and specific detection of E. coli. Caseinenzymic hydrolysate provides essential nutrients. Lactose is a fermentablecarbohydrate. Sodium chloride maintains the osmotic equilibrium. The medium’sstrong buffering system controls pH during fermentative activity, while bilesalts inhibit gram‑positive bacteria,particularly Bacillus species and fecal streptococci, enhancingselectivity for E. coli.

Application

MUG EC broth is used as a selective and differential medium for thedetection and confirmation of Escherichia coli in water, food, andenvironmental samples, often within coliform testing schemes. Bile salts andincubation at elevated temperature (e.g., ~44.5 °C as per standard EC methods)inhibit many non‑coliform bacteria whileallowing growth of coliforms, and the MUG reagent enables differentiation of E.coli from other coliforms by fluorescence. This makes the broth suitablefor regulatory and quality-control analyses where specific enumeration orconfirmation of E. coli is required, including drinking water, recreationalwater, wastewater, and various food matrices.

Features and Benefits

Features:

• High-Quality Standards: NutriSelect^® Plus powdered culture media are made from carefully selected raw materials, ensuring consistent quality.
• Wide Range of Applications: Suitable for various microbiological needs, including academic research, food and beverage testing, and pharmaceutical applications.
• Regulatory Compliance: Products meet industry standards and are compliant with ISO 9001 quality assurance.
• Innovative Formulations: Includes novel media types such as non-animal-derived alternatives, synthetically defined media, and chromogenic media.

Benefits:

• Convenience and Safety: Easy-to-use powdered format with minimal clumping and excellent solubility, even in warm or humid conditions.
• Sustainability: Reduced risks associated with fine dust and toxic substance inhalation, ensuring a safer work environment. ·
• Extended Shelf Life: Products have a prolonged shelf life of up to five years.
• High Reproducibility: Ensures consistent results across batches, exceeding regulatory demands.
• Comprehensive Portfolio: A vast selection of over 350 products tailored to meet diverse microbiological testing needs.

Preparation Note

Suspend 37 g of MUG EC Broth in 1000 ml of distilled water. Dispense in tubes containing inverted Durham′s tubes. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 12-15 minutes.

Other Notes

Ingredients (g/L)
Casein enzymatic hydrolysate, 20.00
Lactose, 5.00
Bile salts mixture, 1.50
Dipotassium phosphate, 4.00
Monopotassium phosphate, 1.50
Sodium chloride, 5.00
4-Methylumbeliferyl β-D-glucuronide (MUG), 0.05

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable