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ABN91

Sigma-Aldrich

Anti-NeuN Antibody

from chicken, purified by affinity chromatography

Synonym(s):

RNA binding protein fox-1 homolog 3, Fox-1 homolog C

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

chicken

Quality Level

100
200

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, rat

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgY

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

General description

NeuN (RNA binding protein fox-1 homolog 3; Fox-1 homolog C) is a RNA-binding protein found exclusively in the nuclei of neuronal cells. It is a member of the evolutionarily conserved Fox-1 family and is mainly involved in splicing of RNA.

Specificity

Other homologies: Human (94% sequence homology).
This antibody recognizes the N-terminus of NeuN.

Immunogen

Epitope: N-terminus
GST-tagged recombinant protein corresponding to the N-terminus of mouse NeuN.

Application

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected NeuN in rat E18 cortex cells.

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected NeuN in mouse brain, mouse hippocampus, and mouse frontal cortex tissues.
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience
This Anti-NeuN Antibody is validated for use in IHC(P), Western Blotting, ICC for the detection of NeuN.

Quality

Evaluated by Western Blot in mouse brain E16 tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected NeuN on 10 µg of mouse brain E16 tissue lysate.

Target description

~45 kDa observed. Uncharacterized bands appear at ~40 and ~50 kDa in some lysates.

Physical form

Affinity purified
Purified chicken polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse brain E16 tissue lysate

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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