AB745

Anti-Collagen Type I Antibody

Chemicon^®, from rabbit

• Synonym(s): Anti-CAFYD, Anti-EDSARTH1, Anti-EDSC, Anti-OI1, Anti-OI2, Anti-OI3, Anti-OI4
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: purified immunoglobulin (Semi-purified)
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: human
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable; immunohistochemistry: suitable; western blot: suitable
• suitability: not suitable for immunohistochemistry (Paraffin)
• NCBI accession no.: NP_000079
• UniProt accession no.: P02452
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... COL1A1(1277)

Description

Specificity

Recognizes Human collagen type I. Some cross reactivity with other proteins.

Immunogen

Human placental collagen type I

Application

ELISA on human collagen type I: 1:4,000.

Indirect microimmunofluorescent visualization of collagen type I on cryostat sections of human tissue or cultured cells: 1:10-1:40.

Western blot 1:200

Not recommended for use in paraffin treated tissues.

Optimal working dilutions must be determined by the end user.

Research Category
Cell Structure

Research Sub Category
ECM Proteins

This Anti-Collagen Type I Antibody is validated for use in ELISA, IH, WB for the detection of Collagen Type I.

Physical form

Ammonium sulfater precipitation followed by DEAE chromatography, and immunodeletion

Format: Semi-Purified

Immunoglobulin fraction, prepared by ammonium sulfate precipitation and chromatography on DEAE-cellulose, of antiSerum cross-absorbed over immobilized human albumin, immunoglobulin and collagen types III, IV and V.

Product is liquid in 0.15M Sodium Chloride, 10mM Sodium Phosphate pH 7.5 with 0.1% Mannitol and 0.1% Dextran as stabilizers. No preservative.

Storage and Stability

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable