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227040

Sigma-Aldrich

Anti-Cholera Toxin, B-Subunit Goat pAb

lyophilized, Calbiochem®

Synonym(s):

Anti-Cholera Antibody, Cholera Toxin Detection, Goat Anti-Cholera Toxin

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.43

biological source

goat

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized

contains

≤0.1% sodium azide as preservative

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

isotype

IgG

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

General description

Goat polyclonal antibody supplied as lyophilized undiluted serum. Recognizes cholera toxin B-subunit.
Recognizes the B-subunit of cholera toxin.
This Anti-Cholera Toxin, B-Subunit Goat pAb is validated for use in Immunoblotting, Frozen Sections, Immunocytochemistry, ELISA for the detection of Cholera Toxin, B-Subunit.

Immunogen

Vibrio cholerae
non-denatured Cholera Toxin, B-subunit

Application

Immunoblotting (see application references)

Frozen Sections (1:4000; see application references)

Immunocytochemistry (1:200; see application references)

ELISA (1:10,000; see application references)

Warning

Toxicity: Standard Handling (A)

Physical form

Undiluted serum.

Reconstitution

Reconstitute in 100 µl dH₂O. Further dilute with aqueous buffers, such as PBS.

Other Notes

Antibody should be titrated for optimal results in individual systems.
Vadolas, J., et al. 1995. Eur. J. Immunol. 25, 969.
Craig, J.P. 1971. Microbial Toxins 2A, 189.

Selected Citations
Peters, I., et al. 2009. Biochim. Biophys. Acta1788, 964.
Balasubramanian, N., et al. 2007. Nature Cell Biology9, 1381.
Nemchinov, L.G., Et al. 2000. Arch. Virol.145, 2557.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

11 - Combustible Solids

WGK

WGK 1


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Nagaraj Balasubramanian et al.
Nature cell biology, 9(12), 1381-1391 (2007-11-21)
Integrin-mediated adhesion regulates membrane binding sites for Rac1 within lipid rafts. Detachment of cells from the substratum triggers the clearance of rafts from the plasma membrane through caveolin-dependent internalization. The small GTPase Arf6 and microtubules also regulate Rac-dependent cell spreading
L G Nemchinov et al.
Archives of virology, 145(12), 2557-2573 (2001-02-24)
Hepatitis C virus (HCV) is a major cause of acute and chronic hepatitis with over 180 million cases worldwide. Vaccine development for HCV has been difficult. Presently, the virus cannot be grown in tissue culture and there is no vaccine
Jing Du et al.
Cerebrospinal fluid research, 6, 3-3 (2009-03-19)
It has been shown that distal cerebrospinal fluid-contacting neurons (dCSF-CNs) exist near the ventral midline of the midbrain aqueduct and also in the grey matter of the inferior third ventricle and the fourth ventricle floor in the superior segment of
J Müthing et al.
Glycoconjugate journal, 14(1), 19-28 (1997-01-01)
In this study the comparative TLC immunostaining investigation of neutral GSLs and gangliosides from human skeletal and heart muscle is described. A panel of specific polyclonal and monoclonal antibodies as well as the GM1-specific choleragenoid were used for the overlay
Annabel Guichard et al.
Cell host & microbe, 14(3), 294-305 (2013-09-17)
Cholera toxin (CT), a virulence factor elaborated by Vibrio cholerae, is sufficient to induce the severe diarrhea characteristic of cholera. The enzymatic moiety of CT (CtxA) increases cAMP synthesis in intestinal epithelial cells, leading to chloride ion (Cl(-)) efflux through

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