HPLC Analysis of Norepinephrine and 3,4-Dihydroxyphenylglycol on Discovery^® HS F5

column

Discovery HS F5, 15 cm × 4.6 mm I.D., 5 μm particles (567561-U)

mobile phase

50 mM ammonium formate, pH to 3.0 with formic acid

flow rate

1.0 mL/min

column temp.

35 °C

detector

UV, 266 nm

injection

10 μL

sample

50 μg/mL each (NE and DHPG) in 5% methanol in mobile phase