HPLC Analysis of Fat Soluble Vitamins on Ascentis^® Express RP-Amide, Acetonitrile Mobile Phase

column

Ascentis Express RP-Amide, 15 cm x 4.6 mm I.D., 2.7 μm particles (53931-U)

mobile phase

acetonitrile

flow rate

1.5 mL/min

pressure

1682 psi (116 bar)

column temp.

30 °C

detector

UV, 280 nm

injection

5 μL

sample

0.5 mg/mL tocopherol acetate, alpha-tocopherol, delta-tocopherol and K1, 0.05 mg/mL D2, D3 and retinol acetate, 1.5 mg/mL retinol palmitate, all in ethanol