HPLC Analysis of Diet Soda Spiked with Neotame on Ascentis^® Express RP-Amide

column

Ascentis Express RP-Amide, 3 cm x 4.6 mm I.D., 2.7 μm particles (53921-U)

mobile phase

[A] 100 mM ammonium acetate, pH 5.6 with acetic acid; [B] water; [C] acetonitrile

gradient

20% A constant; 75 to 20% B, 5 to 60% C in 1 min; held at 20% B, 60% C for 0.1 min

flow rate

3 mL/min

pressure

3336 psi (230 bar)

column temp.

40 °C

detector

UV, 214 nm

injection

1 mL/min

sample

diet soda spiked with neotame 350 μg/mL