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HPLC Analysis of Carotenoids by Normal Phase Chromatography on Ascentis® Si

HPLC Analysis of Carotenoids by Normal Phase Chromatography on Ascentis® Si application for HPLC

Matériaux

Colonne analytique

Réf. du produit
Description
Tarif

581512-U

Colonne HPLC Ascentis® Si

5 μm particle size, L × I.D. 15 cm × 4.6 mm

Composant de phase mobile

Réf. du produit
Description
Tarif

270490

2-Propanol

99.5%, HPLC grade

Étalon

Réf. du produit
Description
Tarif

X6250

Xanthophyll

from marigold

SML0982

Astaxanthin

≥97% (HPLC), from Blakeslea trispora

CONDITIONS

column

Ascentis Si, 15 cm x 4.6 mm I.D., 5 μm particles (581512-U)

mobile phase

[A] hexane; [B] ethyl acetate; [C] isopropanol; (85:10:5, A:B:C)

flow rate

1.0 mL/min

column temp.

30 °C

detector

UV, 450 nm

injection

10 μL

sample

50-100 μg/mL hexane

Description

Remarque sur l'analyse

Ascentis silica is used in the analysis of naturally occurring carotenoid pigments in the form of alcohols, xanthophyll and astaxanthin.

Informations légales

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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Related Product Categories

HPLC ColumnsGC Columns & AccessoriesTLC Plates & AdsorbentsSolid Phase Microextraction (SPME)Solid Phase Extraction & QuEChERS

Related Applications

Small Molecule HPLCLarge Molecule HPLCGas Chromatography (GC)TLCSolid Phase Microextraction (SPME)Solid Phase Extraction (SPE)QuEChERS