GC Analysis of Polynuclear Aromatic Hydrocarbons (PAHs) in Grilled Hamburger on SPB®-608 (20 m x 0.18 mm I.D., 0.18 μm) after QuEChERS Cleanup using Supel™ QuE Z-Sep, Fast GC Analysis
CONDITIONS
sample preparation
Dispersive (QuEChERS)
sample/matrix
grilled hamburger spiked with PAHs at 100 ng/g
extraction tube
Sodium chloride/magnesium sulfate (55294-U)
extraction process
add 25 mL acetonitrile, contents of unbuffered salt tube (55294-U); shake for 5 minutes; centrifuge for 5 minutes at 3200 rpm
clean-up tube
Supel QuE Z-Sep tube (55403-U)
clean-up process
transfer 3 mL of acetonitrile layer into a Supel QuE Z-Sep cleanup tube (55403-U); shake 1 minute; centrifuge for 3 minutes at 3400 rpm; remove 1 mL supernatant for GC-MS analysis
column
SPB-608, 20 m x 0.18 mm I.D. x 0.18 μm
oven
60 °C (1 min), 25 °C/min to 275 °C, 10 °C/min to 300 °C (13 min)
inj. temp.
265 °C
detector
MS-SIM
MSD interface
300 °C
carrier gas
helium, 1.5 mL/min constant flow
injection
1 μL splitless (1 min.)
liner
4 mm I.D. FocusLiner™ with taper
sample
grilled hamburger extract
Description
Remarque sur l'analyse
Sample pretreatment: Place 5 g ground hamburger sample into 50 mL centrifuge tube (55248-U). Spike with 100 ng/g target PAHs and internal standards. Let sit 10 min. Add 5 mL water and homogenize.
This application demonstrates the use of dispersive SPE or QuEChERS for the extraction and cleanup of grilled hamburger for GC-MS analysis of polynuclear aromatic hydrocarbons (PAHs). Z-Sep sorbent was used in the cleanup step and found to reduce background and yield recoveries >80% for a majority of the PAHs tested.
Autres remarques
Informations légales
FocusLiner is a trademark of SGE Analytical Science Pty Ltd.
SPB is a registered trademark of Merck KGaA, Darmstadt, Germany
Supel is a trademark of Sigma-Aldrich Co. LLC