Accéder au contenu
Merck
Toutes les photos(4)

Documents

XNAPG

Sigma-Aldrich

SYBR® Green Extract-N-Amp™ Plant PCR Kit

sufficient for 100 preparations

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(4)

About This Item

Code UNSPSC :
41106303
Nomenclature NACRES :
NA.55

Utilisation

sufficient for 100 preparations
 sufficient for 100 reactions

Niveau de qualité

200

Caractéristiques

dNTPs included
hotstart

Technique(s)

qPCR: suitable

Méthode de détection

SYBR® Green

Conditions d'expédition

wet ice

Température de stockage

−20°C

Vous recherchez des produits similaires ? Visite Guide de comparaison des produits

Description générale

SYBR® Green Extract-N-Amp™ Plant PCR Kit is recommended for fast and reliable direct PCR from a wide range of plant leaf tissue.
The SYBR Green Extract-N-Amp Plant PCR Kit contains all the reagents needed for rapid extraction of genomic DNA from plant leaf tissue coupled with direct polymerase chain reaction (PCR) amplification and real-time detection. The need for freezing plant tissue in liquid nitrogen or mechanical disruption and complete genomic DNA purification has been eliminated.
Two solutions provided in the kit combine to release genomic DNA from leaf tissue and neutralize substances inhibitory to PCR. The genomic DNA released into solution serves as a template for the SYBR Green Extract-N-Amp PCR ReadyMix™. This ReadyMix, which contains SYBR Green fluorescent dye, Taq, JumpStart antibody, dNTPs and MgCl2, is specially formulated to amplify target sequences in the cellular lysate.

Caractéristiques et avantages

  • Single-step extraction of plant genomic DNA for polymerase chain reaction (PCR) in less than 15 minutes
  • No freezing, mechanical disruption, organic extraction, column purification, or precipitation required
  • Specially formulated PCR ReadyMix for use with extract
  • Hot Start antibody for highly specific PCR amplification of genomic DNA
  • No loading buffers or tracking dyes required for gel analysis
  • SYBR® Green I dye acts as a nonspecific reporter for real-time PCR
  • SYBR® Green I dye is inexpensive, easy to use, and highly sensitive
  • Compatible with high-throughput requirements for genetic analysis of plants
  • No long enzymatic digestions
  • Extract stable at 4°C for at least 6 months

Autres remarques

The protocol uses a 20 μl PCR reaction.

Informations légales

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Life Technologies

Composants de kit également disponibles séparément

Réf. du produit
Description
FDS
  • E7526Extraction SolutionFDS
  • D5688Dilution SolutionFDS
  • S4320SYBR® Green Extract-N-Amp™ PCR ReadyMix™FDS

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

W1754

Eau, PCR Reagent

P6097

Precast Agarose Gels, 24 wells (2 × 12-well portrait orientation)

XNAP

Kit de PCR REDExtract-N-Amp™ Plant, sufficient for 100 extractions, sufficient for 100 amplifications

T4415

Tampon Tris-borate-EDTA, BioReagent, suitable for electrophoresis, 10× concentrate

T9525

Tampon Tris-borate-EDTA, working solution

T6400

Tampon Tris-borate-EDTA, 5× Concentrate

P9577

PCR 50 - 2,000 bp Marker, for electrophoresis of PCR fragments

XNAPE

Kit de PCR REDExtract-N-Amp™ Plant, sufficient for 100 extractions, sufficient for 500 amplifications

XNAP2

Extract-N-Amp™ Plant PCR Kit, sufficient for 100 extractions, sufficient for 100 amplifications

XNAR

Extract-N-Amp™ Plant PCR Kit, sufficient for 1000 extractions, sufficient for 1000 amplifications

XNAPR

Kit de PCR REDExtract-N-Amp™ Plant, sufficient for 1000 extractions, sufficient for 1000 amplifications

XNAPS

Kit de PCR REDExtract-N-Amp™ Plant, sufficient for 10 extractions, sufficient for 10 amplifications

R4775

ReadyMix PCR REDExtract-N-Amp™™, Ready-to-use 2X PCR Master Mix with Loading Dye

E3004

ReadyMix pour PCR Extract-N-Amp™™, Amplifications to support Extract-N-Amp Plant and Extract-N-Amp Tissue

E8155

Extract-N-Amp™ PCR Diluent, colorless, storage temp.:2-8°C

D5688

Dilution Solution, Extract-N-Amp Plant Dilution Solution

S4320

SYBR® Green Extract-N-Amp™ PCR ReadyMix™, Amplifications to support Extract-N-Amp Plant and Extract-N-Amp Tissue

E0781

Extract-N-Amp™ PCR ReadyMix™ with qPCR Reference dye, 125 mL sufficient for 10000 amplifications, hotstart, dNTPs included

Pictogrammes

Exclamation markEnvironment
GHS07,GHS09

Mention d'avertissement

Warning

Mentions de danger

H315,H317,H319,H411

Classification des risques

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Trenis D Palmer et al.
Journal of visualized experiments : JoVE, (51)(51), doi:10-doi:10 (2011-06-16)
During metastasis cancer cells disseminate from the primary tumor, invade into surrounding tissues, and spread to distant organs. Metastasis is a complex process that can involve many tissue types, span variable time periods, and often occur deep within organs, making

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique