T9378

Trypsin inhibitor

crude powder

• Synonyme(s) : Trypsin Inhibitor from lima beans
• Numéro CAS: 9035-81-8
• Numéro CE : 232-906-9
• Numéro MDL: MFCD00082102
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.77

Propriétés

• Nom du produit: Trypsin inhibitor from Phaseolus limensis (lima bean), Type II-L, crude powder
• Source biologique: Phaseolus limensis (lima bean)
• Niveau de qualité: 200
• Type: Type II-L
• Forme: crude powder
• Poids mol.: 9 kDa
• Solubilité: 0.067 M sodium phosphate buffer, pH 7.6: 1 mg/mL
• Température de stockage: 2-8°C

Description

Application

Trypsin inhibitor from Phaseolus limensis (lima bean) has been used:

• in the inhibition of trypsin in human plasma
• in the termination of proteolytic digestion in rabbit skeletal muscles
• in the inhibition of salivary glands enzyme extract from Lygus lineolaris
• in the inhibition of proteolytic activity in Agave tequilana enzyme extract

Trypsin inhibitor has been used as an affinity ligand for isolation of elastase-type enzymes.

Actions biochimiques/physiologiques

Trypsin inhibitor from lima beans belongs to Bowman-Birk family of protease inhibitors. It is 9 kDa in molecular weight and contains nine disulfide bridges and is highly thermostable. It has trypsin and chymotrypsin binding subdomains.

Propriétés physiques

Monomer has an apparent molecular weight of approx. 9,000 Da but undergoes a concentration and pH-dependent dimerization.

Définition de l'unité

One trypsin unit will produce a ΔA₂₅₃ of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 mL, 1 cm light path.

Notes préparatoires

Prepared by a modification of the method of Tauber, H., et al., J. Biol. Chem., 179, 1155 (1949) (modified).

Remarque sur l'analyse

One mg will inhibit ≥0.8 mg of trypsin with activity of approx. 10,000 BAEE units per mg protein.

Autres remarques

View more information on Trypsin Inhibitor.

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H317,H334
• Conseils de prudence: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Classification des risques: Resp. Sens. 1 - Skin Sens. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)