T3526

Anti-Tubulin antibody produced in rabbit

whole antiserum

• Synonyme(s) : Anti-K-ALPHA-1
• Numéro MDL: MFCD00145921
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41
• Conjugué: unconjugated
• application: IF; WB
• Clone: polyclonal
• Espèces réactives: chicken, mouse
• citations: 62
• Technique(s): indirect immunofluorescence: 1:80 using cultured fibroblasts; western blot: 1:200 using chicken gizzard extract or mouse brain extract

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 200
• Conjugué: unconjugated
• Forme d'anticorps: whole antiserum
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Contient: 15 mM sodium azide
• Espèces réactives: chicken, mouse
• Technique(s): indirect immunofluorescence: 1:80 using cultured fibroblasts; western blot: 1:200 using chicken gizzard extract or mouse brain extract
• Numéro d'accès UniProt: P68363
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: mouse ... Tuba1b(22143) , Tubb2a(22151) , Tubb2b(73710) , Tubb2c(227613)

Description

Description générale

Microtubules are composed of tubulin and several additional proteins designated microtubule associated proteins (MAPs). Tubulin is a heterodimer which consists of an α-chain and a β-chain, both of which have a molecular weight of approximately 55,000. Microtubules form an elaborate cytoplasmic network during interphase and are reorganized into the mitotic spindle during cell division.

Tubulin is an extremely conserved and negatively charged protein. It is expressed in all eukaryotic cells, but mainly in brains.

Spécificité

The product stains tubulin in cultured chicken fibroblasts or 3T3 mouse fibroblasts. Additional bands may be stained in immunoblotting.

Immunogène

microtubule proteins from brains of 13 day old chicken embryos

Application

Anti-Tubulin antibody produced in rabbit has been used in immunoblotting and indirect immunofluorescence.

Actions biochimiques/physiologiques

αβ-tubulin heterodimers helps in formation of microtubules. Tubulin dimers exhibits GTPase activity, assembly and disassembly properties. It facilitates the construction of the flagellar apparatus.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)