STR1
Enhanced Avian First Strand Synthesis Kit
Components for cDNA synthesis with enhanced AMV reverse transcriptase
Synonyme(s) :
Reverse Transcriptase cDNA synthesis kit, eAMV-RT
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About This Item
Code UNSPSC :
12352200
Nomenclature NACRES :
NA.55
Produits recommandés
Niveau de qualité
Utilisation
sufficient for 50 reactions
kit sufficient for 50 reactions
Caractéristiques
dNTPs included
hotstart: no
Technique(s)
RT-PCR: suitable
Couleur
colorless
Entrée
purified RNA
Conditions d'expédition
dry ice
Température de stockage
−20°C
Description générale
Enhanced Avian First Strand Synthesis Kit utilizes a highly purified avian myeloblastosis virus reverse transcriptase (eAMV-RT) that offers superior performance in comparison to standard AMV-RT or standard Moloney murine leukemia virus reverse transcriptase (MMLV-RT). This exceptionally robust eAMV-RT has an enhanced ability to transcribe through difficult secondary structure at elevated temperatures (up to 65 °C) making it the ideal enzyme for producing high quality full-length cDNA from total RNA or poly(A)+ RNA.
Sigma′s Enhanced Avian RT First Strand Synthesis Kit also provides random nonamers (9-mers) and anchored oligo (dT)23 primers since gene-specific primers may not always be useful or possible. The anchored oligo has 23 thymidine residues and one G, C, or A residue (the anchor). The anchor ensures that the oligo (dT) primer binds at the very beginning of the message and that there is not a long region of the unusable sequence. With this kit, a dependable cDNA is generated that can be used for various downstream applications, including PCR.
Application
Enhanced Avian First Strand Synthesis Kit has been used for cDNA synthesis, which is intended for use in a real-time reverse transcription-polymerase chain reaction (RT-PCR).
Définition de l'unité
One unit incorporates one nanomole of TMP into TCA-precipitable material in 10 minutes using polyadenylic acid as template and oligo(dT)12-18 as primer.
Informations légales
Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.
Composants de kit seuls
Réf. du produit
Description
- Enhanced Avian Reverse Transcriptase 1000 U
Produit(s) apparenté(s)
Code de la classe de stockage
10 - Combustible liquids
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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