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Key Documents

SRP6233

Sigma-Aldrich

Relaxin-3 human

≥98% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder

Synonyme(s) :

H3 relaxin, INSL7, Insulin-like peptide-7

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About This Item

Code UNSPSC :
12352202
Nomenclature NACRES :
NA.32

product name

Relaxin-3 human, recombinant, expressed in E. coli, ≥98% (SDS-PAGE)

Source biologique

human

Produit recombinant

expressed in E. coli

Pureté

≥98% (SDS-PAGE)

Forme

lyophilized powder

Poids mol.

5.5 kDa

Conditionnement

pkg of 10 μg
pkg of 50 μg

Impuretés

<1 EU/μg endotoxin (LAL test)

Numéro d'accès UniProt

Q8WXF3

Conditions d'expédition

wet ice

Température de stockage

−20°C

Informations sur le gène

human ... RLN3(117579)

Description générale

RLN3 (relaxin-3) is a relaxin peptide, belonging to the insulin superfamily, with each member containing two peptide subunits linked by three disulfide bridges. This protein shows a restricted expression pattern in the brain, mainly in lateral periaqueductal gray, nucleus incertus, and central gray regions in the brainstem. This protein is highly conserved across a range of species from fish to mammals.

Actions biochimiques/physiologiques

RLN3 (relaxin-3) functions as a ligand for the leucine-rich repeat-containing G-protein-coupled receptor (LGR), LGR7. It also acts as a ligand for orphan receptor GPCR135, or SALPR (somatostatin- and angiotensin-like peptide receptor). RLN3 acts on LGR7 resulting in stimulation of adenylate cycle; RLN3 acts on GPCR135 to prevent activation of adenylate cyclase. The RLN3–GPCR135 signaling pathway, functions alongside GABA neurotransmitter in specific brain areas, and has regulatory effects on putative GABA neuronal networks. It also interacts with pathways containing CRF (corticotrophin releasing hormone)-like peptides and their receptors. CRF-like peptides and their receptors are implicated in stress-induced autonomic and behavioral changes. RLN3–GPCR135 system is overall involved in arousal, learning and memory, stress, and locomotor activity.

Forme physique

Sterile filtered through a 0.2 micron filter. Lyophilized with no additives.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Identification of relaxin-3/INSL7 as a ligand for GPCR142.
Liu C, et al.
The Journal of Biological Chemistry, 278(50), 50765-50770 (2003)
Relaxin-3 in GABA projection neurons of nucleus incertus suggests widespread influence on forebrain circuits via G-protein-coupled receptor-135 in the rat.
Ma S, et al.
Neuroscience, 144(1), 165-190 (2007)
Central relaxin-3 administration causes hyperphagia in male Wistar rats.
McGowan BM, et al.
Endocrinology, 146(8), 3295-3300 (2005)

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