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Key Documents

SRP6014

Sigma-Aldrich

HGF human

recombinant, expressed in CHO cells, ≥97% (SDS-PAGE), ≥97% (HPLC)

Synonyme(s) :

F-TCF, HGF, HGFB, Hepatopoietin (HPTA), Scatter Factor (SF)

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About This Item

Numéro CAS:
953836-17-4
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.32

Source biologique

human

Produit recombinant

expressed in CHO cells

Pureté

≥97% (HPLC)
≥97% (SDS-PAGE)

Forme

lyophilized

Poids mol.

80.017 kDa

Conditionnement

pkg of 1 mg
pkg of 10 μg

Technique(s)

cell culture | embryo: suitable

Impuretés

Endotoxin, tested

Numéro d'accès NCBI

P14210

Numéro d'accès UniProt

P14210

Conditions d'expédition

wet ice

Température de stockage

−20°C

Informations sur le gène

human ... HGF(3082)

Catégories apparentées

Description générale

Hepatocyte growth factor (HGF) is a multifunctional growth factor. HGF Human Recombinant produced in Chinese hamster ovary (CHO) is a heterodimer, non-glycosylated, polypeptide chain consisting of an α-chain of 463 amino acids and β-chain of 234 having a total molecular mass of 80.017kDa. The gene encoding this protein is localized on human chromosome 7q21.1. The HGF is purified by proprietary chromatographic techniques.

Actions biochimiques/physiologiques

Hepatocyte growth factor (HGF) regulates both cell growth and cell motility. It exerts a strong mitogenic effect on hepatocytes and primary epithelial cells. HGF synergizes with interleukin-3 and granulocyte-macrophage colony-stimulating factor (GM-CSF) to stimulate colony formation of hematopoietic progenitor cells in vitro and may, therefore, also modulate hematopoiesis. It is expressed in brain tumors.

Forme physique

The protein was lyophilized from a concentrated (1 mg/ml) solution containing PBS pH 7.4 containing 0.05% Tween 20 and 100 mM L-arginine.

Reconstitution

Centrifuge the vial prior to opening. It is recommended to reconstitute the lyophilized Hepatocyte Growth Factor (HGF) in sterile 18 MΩcm-1 H2O not less than 100 μg/mL, which can then be further diluted to other aqueous solutions.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

HGF: a multifunctional growth factor controlling cell scattering.
Stella MC and Comoglio PM
The International Journal of Biochemistry & Cell Biology, 31(12), 1357-1362 (1999)
Rajani Kanteti et al.
PloS one, 9(9), e105919-e105919 (2014-09-16)
Malignant pleural mesothelioma (MPM) is an aggressive disease with a poor prognosis. Studies have shown that both MET and its key downstream intracellular signaling partners, PI3K and mTOR, are overexpressed in MPM. Here we determined the combinatorial therapeutic efficacy of
Synergistic effects of hepatocyte growth factor on human cord blood CD34+ progenitor cells are the result of c-met receptor expression.
Goff JP
Stem Cells, 14(5), 592-602 (1996)
Scatter factor/hepatocyte growth factor in brain tumor growth and angiogenesis.
Abounader R and Laterra J
Neuro-Oncology, 7(4), 436-451 (2005)
Hiroshi Kusunoki et al.
Hypertension research : official journal of the Japanese Society of Hypertension, 37(6), 500-506 (2014-03-14)
The paracrine loop involving inflammatory cytokines between adipocytes and macrophages establishes a vicious cycle that aggravates pro-inflammatory changes in adipose tissue. The serum level of hepatocyte growth factor (HGF) is increased in metabolic syndrome, but whether HGF is beneficial or
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