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Key Documents

SRP3138

Sigma-Aldrich

PDGF-BB human

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Synonyme(s) :

Glioma-derived growth factor (GDGF), Osteosarcoma-derived Growth Factor (ODGF), Platelet-Derived Growth Factor-BB

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About This Item

Code UNSPSC :
12352202
eCl@ss :
32190102
Nomenclature NACRES :
NA.32

Source biologique

human

Produit recombinant

expressed in E. coli

Pureté

≥98% (HPLC)
≥98% (SDS-PAGE)

Forme

lyophilized

Puissance

1.0-3.0 ng/mL ED50

Poids mol.

24.3 kDa

Conditionnement

pkg of 10 μg

Technique(s)

cell culture | mammalian: suitable

Impuretés

<0.1 EU/μg endotoxin, tested

Couleur

white to off-white

Numéro d'accès UniProt

Conditions d'expédition

wet ice

Température de stockage

−20°C

Informations sur le gène

human ... PDGFB(5155)

Description générale

PDGFs (platelet-derived growth factors) are disulfide-linked dimers consisting of two 12.0-13.5kDa polypeptide chains, designated PDGF-A and PDGF-B chains. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-α and PDGFR-β. PDGFR-α is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-β interacts with only PDGF-BB and PDGF-AB. The gene PDGF-B is mapped to human chromosome 22q13.1. Recombinant human PDGF-BB is a 24.3kDa disulfide-linked homodimer of two B chains (218 total amino acids).

Application

PDGF-BB human has been used in the cell viability assay. It has been used as a stimulator in PDGFRα (platelet-derived growth factor receptor α)-positive HTPCs (human testicular peritubular cells).

Actions biochimiques/physiologiques

The three naturally occurring PDGFs (platelet-derived growth factors); PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet α-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. PDGF-B along with PDGFR-β participates in vascular maturation. It brings smooth muscle cells and pericytes to growing vessels. Heterozygous mutation in PDGF-B is associated with primary familial brain calcification.

Séquence

SLGSLTIAEP AMIAECKTRT EVFEISRRLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RPTQVQLRPV QVRKIEIVRK KPIFKKATVT LEDHLACKCE TVAAARPVT

Forme physique

Lyophilized from 10 mM Acetic Acid.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.

Pictogrammes

Exclamation mark

Mention d'avertissement

Warning

Mentions de danger

Classification des risques

Eye Irrit. 2 - Skin Irrit. 2

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Human testicular peritubular cells host putative stem Leydig cells with steroidogenic capacity.
Landreh L, et al.
The Journal of Clinical Endocrinology and Metabolism, 99, E1227-E1235 (2014)
Dovitinib preferentially targets endothelial cells rather than cancer cells for the inhibition of hepatocellular carcinoma growth and metastasis.
Chen ZY, et al.
Journal of Translational Medicine, 10, 245-245 (2012)
Decorin inhibition of PDGF-stimulated vascular smooth muscle cell function: potential mechanism for inhibition of intimal hyperplasia after balloon angioplasty.
Nili N, et al.
The American Journal of Pathology, 163, 869-878 (2003)
Platelets in inflammation and atherogenesis.
Gawaz M, et al.
The Journal of Clinical Investigation, 115, 3378-3384 (2005)
Thomas Daubon et al.
Nature communications, 10(1), 1146-1146 (2019-03-10)
We undertook a systematic study focused on the matricellular protein Thrombospondin-1 (THBS1) to uncover molecular mechanisms underlying the role of THBS1 in glioblastoma (GBM) development. THBS1 was found to be increased with glioma grades. Mechanistically, we show that the TGFβ

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