SRP3073
IL-12 human
recombinant, expressed in CHO cells, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Synonyme(s) :
CTL maturation factor (TCMF),, Cytotoxic lymphocyte maturation factor (CLMF), NKSF, TSF
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About This Item
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.32
Produits recommandés
Source biologique
human
Produit recombinant
expressed in CHO cells
Pureté
≥98% (HPLC)
≥98% (SDS-PAGE)
Forme
lyophilized
Puissance
≤1.0 ng/mL ED50
Poids mol.
40 kDa
Conditionnement
pkg of 10 μg
Technique(s)
cell culture | mammalian: suitable
Impuretés
<0.1 EU/μg endotoxin, tested
Couleur
white to off-white
Conditions d'expédition
wet ice
Température de stockage
−20°C
Informations sur le gène
human ... IL12A(3592)
Description générale
IL-12 (interleukin 12) is produced by activated monocytes/macrophage cells, B lymphocytes and connective tissue type mast cells. Recombinant Human IL-12 is a 75kDa heterodimeric glycoprotein consisting of disulfide-linked 35kDa (p35) and 40kDa (p40) subunits (503 total amino acid residues).
Actions biochimiques/physiologiques
IL-12 (interleukin 12) is a proinflammatory cytokine and plays an important role in protection against pathogens. IL-12 is a potent regulator of cell mediated immune responses and it induces IFN (interferon)-γ production by NK (natural killer) and T cells. Among its biological activities IL-12 promotes the growth and activity of activated NK, CD4+ and CD8+ cells and induces the development of IFN-γ producing Th1 (T helper) cells. Polymorphisms in the IL-12 gene are associated with risk of rheumatoid arthritis. It also exhibits anti-tumor activities by enhancing Th1 adaptive immunity and cytotoxic T lymphocyte functions.
Séquence
p35 Subunit: RNLPVATPDP GMFPCLHHSQ NLLRAVSNML QKARQTLEFY PCTSEEIDHE DITKDKTSTV EACLPLELTK NESCLNSRET SFITNGSCLA SRKTSFMMAL CLSSIYEDLK MYQVEFKTMN AKLLMDPKRQ IFLDQNMLAV IDELMQALNF NSETVPQKSS LEEPDFYKTK IKLCILLHAF RIRAVTIDRV MSYLNAS p40 Subunit: IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK NASISVRAQD RYYSSSWSEW ASVPCS
Forme physique
Lyophilized from 0.5x PBS, pH 7.2.
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in 1x PBS, pH 7.2-7.4 to a concentration of 0.2 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Les clients ont également consulté
An absolute and restricted requirement for IL-12 in natural killer cell IFN-gamma production and antiviral defense. Studies of natural killer and T cell responses in contrasting viral infections.
Orange JS and Biron CA
Journal of Immunology, 156, 1138-1142 (1996)
Up-regulation of IL-12 expression in patients with chronic hepatitis B is mediated by the PI3K/Akt pathway.
Wang HW, te al.
Molecular and Cellular Biochemistry, 407, 135-142 (2015)
IL-12 induces the production of IFN-gamma by neonatal human CD4 T cells.
Wu CY, et al.
Journal of Immunology, 151, 1938-1949 (1993)
Association between polymorphisms of interleukin 12 and rheumatoid arthritis associated biomarkers in a Chinese population.
Shen L, et al.
Cytokine, 76, 363-367 (2015)
Martin J Page et al.
British journal of haematology, 180(1), 110-117 (2017-11-17)
Inflammation, with its associated inflammatory molecules, is integral to most chronic diseases, including the various cardiovascular diseases. Interleukin 12 (IL12) is one of the inflammatory cytokines that is upregulated during inflammation; however, we know very little about its exact effect
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
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