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Key Documents

SRP0217

Sigma-Aldrich

SHP-2 Active human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

Synonyme(s) :

Noonan syndrome 1, PTP-1D, PTP2C, PTPN11, SHP2

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About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
NA.32

Source biologique

human

Produit recombinant

expressed in E. coli

Pureté

≥90% (SDS-PAGE)

Forme

aqueous solution

Poids mol.

59.5 kDa

Conditionnement

pkg of 20 μg

Conditions de stockage

avoid repeated freeze/thaw cycles

Concentration

0.73 mg/mL

Numéro d'accès NCBI

NM_002834

Numéro d'accès UniProt

Q06124

Conditions d'expédition

dry ice

Température de stockage

−70°C

Informations sur le gène

human ... PTPN11(5781)

Description générale

Protein tyrosine phosphatase, non-receptor type 11 (PTPN11), also known as SHP-2, is a scaffolding protein. It possesses two SH2 domains at the amino terminal, a protein tyrosine phosphatase domain and carboxy-terminal region. The gene encoding SHP-2 is localized on human chromosome 12q24.13.

Application

Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors.
Human active SHP-2 has been used to study the effects of α-lipoic acid and dihydrolipoic acid on the activity of SHP-2 enzyme.

Actions biochimiques/physiologiques

Protein tyrosine phosphatase, non-receptor type 11 (PTPN11) or SHP-2 is involved in signal transduction pathways downstream of growth receptors. It acts as a proto-oncogene. SHP-2 leads to sustained RAS activation by dephosphorylating the RAS-GTPase-activating protein binding site on Grb2-associated binder 1 (Gab1). This protein also nucleates multiple signaling proteins and thus, acts as an adaptor protein. Mutations in the SHP-2 gene have been associated with Noonan syndrome.

Définition de l'unité

One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.

Forme physique

Formulated in 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, and 10 mM glutathione.

Notes préparatoires

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Autres remarques

Amino acids : 224-529

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Hotspots in PTPN11 Gene Among Indian Children With Noonan Syndrome.
Narayanan DL
Indian Pediatrics, 54(8), 638-643 (2017)
Shp2 Plays a Critical Role in IL-6-Induced EMT in Breast Cancer Cells.
Sun X
International Journal of Molecular Sciences, 18(2) (2017)
Genomic Duplication of PTPN11 is an Uncommon Cause of Noonan Syndrome
John M
American Journal of Medical Genetics. Part A (2009)
SHP2 is induced by the HBx-NF-?B pathway and contributes to fibrosis during human early hepatocellular carcinoma development.
Kang HJ
Oncotarget, 8(16) (2017)
Naphak Modhiran et al.
Science translational medicine, 7(304), 304ra142-304ra142 (2015-09-12)
Complications arising from dengue virus infection include potentially fatal vascular leak, and severe disease has been linked with excessive immune cell activation. An understanding of the triggers of this activation is critical for the development of appropriately targeted disease control
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