Name: MISSION<SUP>®</SUP> shRNA Product Offerings
Brand: SIGMA

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<h2>Individual MISSION<sup>®</sup> <span class="h2-lowercase">sh</span>RNA Clones</h2>
<ul>
<li>Our online tool allows you to view clone-specific validation information and build your own clone sets.</li>
<li>The individual clones are available in three formats: bacterial glycerol stock, purified plasmid DNA and lentiviral transduction particles</li>
<li>Locate shRNA clones for your specific gene of interest by clicking 'Search'</li>
<li>Choose from over 315,000 pre-cloned shRNA constructs for both human and mouse.</li>
<li><a href="/FR/en/technical-documents/technical-article/genomics/advanced-gene-editing/shrnas-for-individual-genes">Learn More</a></li>
</ul>


<h3>Create your own panel.<br>
Different gene pathway of interest? Want a more complete list?<br>
Submit a request.</h3>
<ul>
<li>Gene family sets are fully customizable allowing for complete flexibility to add or delete clone or gene content.</li>
</ul>


<h2>MISSION<sup>®</sup> <span class="h2-lowercase">sh</span>RNA Gene Family Sets</h2>
<h3>Choose from 25 Pre-Designed shRNA Gene Panels</h3>
<ul>
<li>All TRC shRNA clones have been methodically mapped to each current gene set by our Bioinformatics team.</li>
<li>Gene family sets for human (22) and mice (3) include popular kinases, phosphatases, GPCRs, tyrosine kinases, and epigenetic regulators.</li>
<li>Any panel can be arrayed in either 96- or 384- well plate formats</li>
<li>All gene family sets can be used at the benchtop for focused target discovery and pathway analysis.</li>
</ul>


<h2>Create Your Own <span class="h2-lowercase">sh</span>RNA Pool</h2>
<h3>Focus on the genes essential to your research by creating your own custom shRNA pool</h3>
<ul>
<li>Create your custom shRNA pool for maximum return of relevant hits</li>
<li>Customize your volume and aliquoting needs to further enhance your ability to rapidly screen multiple cell lines.</li>
<li>Titers and volumes adequate for <i>in vitro, in vivo </i>and xenograft applications.</li>
<li>Partner with Sigma's next-generation sequencing services for data deconvolution and maximum data return from your screening efforts.</li>
</ul>


<h2>Custom MISSION<sup>®</sup> <span class="h2-lowercase">sh</span>RNA</h2>
<h3>Fully customizable shRNA sequence, vector, and lentiviral manufacturing</h3>
<ul>
<li>Select by methods other than puromycin, including neomycin (G418) and FACS via reporters.</li>
<li>Confirm your lentiviral delivery through a fluorescent reporter.</li>
<li>Customize your shRNA sequence.</li>
<li>Eliminate tedious cloning and viral production.</li>
<li>Service includes: shRNA design, cloning, sequence verifications, DNA quantitation, and titer determination.</li>
</ul>
<p><b>Custom Capabilities: Bringing you more flexibility</b></p>
<ul>
<li>Contract manufacturing: Send us your clones for lentiviral production! We accommodates high-throughput projects, private collections, and alternate libraries. Please request a quote or contact if interested.</li>
</ul>


<h3>Pooled shRNA Libraries<br>
Whole genome shRNA screening for a fraction of the cost.</h3>
<ul>
<li>Affordable - Bench top discovery without robotics or liquid handling.</li>
<li>Comprehensive - Trusted TRC (The RNAi Consortium) content with shRNA taregting 20,000+ human or 21,000+ mouse genes including TRC 1.5 and 2.0 (unique and validated) add-ons which are exclusive to Sigma.</li>
<li>Universal - Simplified whole genome RNAi screening in standard or difficult to expand cell lines.</li>
<li>Versatile - Compatible with FACS and most downstream fluorescent assays.</li>
</ul>


<h2>Services</h2>
<h3>Deconvolution of shRNA Pools</h3>
<ul>
<li>Sigma's deconvolution service lets you easily identify the genes that impact your pooled shRNA screen.</li>
<li>Next-generation sequencing of clones gives a precise number of individual clone occurrence within a pooled shRNA sample.</li>
<li>Comprehensive, reproducible results from pooled shRNA screens.</li>
<li>Statistically robust and information-rich data.</li>
<li><a href="/FR/en/technical-documents/technical-article/genomics/advanced-gene-editing/deconvolution-of-pooled-shrna-screens">Learn More</a></li>
</ul>


<h2>Inducible MISSION<sup>®</sup> <span class="h2-lowercase">sh</span>RNA</h2>
<h3>Tight regulation meets great gene silencing</h3>
<ul>
<li>IPTG-inducible shRNA pLKO-vectors allow for fast response and temporally-controlled gene silencing useful for essential or lethal gene knockdown.</li>
<li>Proven to work <i>in vivo </i>and <i>in vitro</i></li>
<li>Single vector systems in 1X LacO and 3X LacO formats for effective inducible knockdown</li>
<li>Available on demand service includes: shRNA design(choose any TRC clone or custom sequence), cloning, sequence verification, DNA quantitation, and titer determination.</li>
</ul>


<h2><span class="h2-lowercase">sh</span>RNA Controls</h2>
<h3>Measure transduction and knockdown efficiency, or monitor off-target effects.</h3>
<ul>
<li>Positive Transduction Controls: Empty Vector, TurboGFP, TagCFP, TagYFP, TagRFP, and TagFP365 Controls</li>
<li>Positive Knockdown Controls: TurboGFP, eGFP, luciferase, B2M, and ARHGDIA controls</li>
<li>Negative Controls: Empty Vector, Non-target, Turbo GFP, eGFP, and luciferase controls</li>
<li><a href="/FR/en/technical-documents/technical-article/genomics/advanced-gene-editing/shrna-controls">Learn More</a></li>
</ul>


<h2>MISSION<sup>®</sup> <span class="h2-lowercase">sh</span>RNA Libraries</h2>
<h3>Arrayed genome-wide shRNA libraries</h3>
<ul>
<li>Most extensive coverage: Pre-cloned genome-wide arrayed libraries contain 245,000+ clone shRNA library targeting 20,000+ human and 21,000+ mouse genes.</li>
<li>High-throughput manufacturing: World class high-throughput viral manufacturing.</li>
<li>Flexible format options: Entire libraries or single gene sets offered in glycerol stock, DNA, or viral format.</li>
<li>Extended Coverage=Average of 5 - 6 clones targeting each gene.</li>
</ul>


<h2>Delivery</h2>
<h3>Produce high-titer lentivirus using the MISSION<sup>® </sup>Lentiviral Packaging Mix</h3>
<ul>
<li>Accelerate viral production by skipping tedious but critical multivariable plasmid optimization.</li>
<li>Produce high titer lentivirus with MISSION<sup>®</sup> shRNA plasmids with the included easy to use protocol.</li>
<li>Safely produce lentivirus utilizing the three plasmid system with self inactivating, long terminal repeat (SIN/LTR) regions.</li>
<li>Choose between small or large scale lentivirus production with our conveniently sized 250 µl or 1.7 ml sizes.</li>
</ul>


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