SEQR
SeqPlex RNA Amplification Kit
For use with high throughput sequencing technologies
Synonyme(s) :
WGA kit, Whole transcriptome amplification
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NACRES:
NA.55
UNSPSC Code:
41121800
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technique(s)
whole genome amplification: suitable
dilution
(WTA)
input
purified RNA
shipped in
wet ice
storage temp.
−20°C
Catégories apparentées
General description
The SeqPlex™ RNA Amplification Kit for whole transcriptome amplification (WTA) is designed to facilitate next-generation sequencing (NGS) from small quantities or from degraded/highly fragmented RNA (e.g. RNA from formalin-fixed paraffin-embedded (FFPE) tissue samples). The SeqPlex kit allows the user to pre-amplify these and other small quantity/highly fragmented RNA samples for input into a NGS workflow. It also facilitates the amplification of non-polyA tailed RNA isolated from tissue, cultured cells, formalin-fixed samples, or serum while maintaining patterns of differential expression found in the unamplified sample.
Application
SeqPlex™ RNA Amplification Kit has been used to amplify poly(A)-selected RNA.
Features and Benefits
- Low quantities of total RNA random priming technology amplifies fragmented or intact RNA from all sources including FFPE and RIP.
- Semi-degenerate library primer design for more complete transcriptome coverage and efficient priming.
- No need to fragment DNA before sequencing.
- Amplifies ds-cDNA in 8 hours or less.
- Compatible with all next generation sequencing platforms except Pacific Bioscience.
Other Notes
SEQR-500RXN is manufactured on-demand. Contact us for more information.
Legal Information
SeqPlex is a trademark of Sigma-Aldrich Co. LLC
Classe de stockage
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 3
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DNA Sequencing Research Group (DSRG): Evaluation of RNA Amplification Kits at Subnanogram Input Amounts of Total RNA for RNA-Seq
Nicolet C, et al.
Journal of biomolecular techniques : JBT, 24, S70-S70 (2013)
Kathryn D Tuttle et al.
Nature communications, 9(1), 2650-2650 (2018-07-10)
During development in the thymus, invariant natural killer T (iNKT) cells commit to one of three major functionally different subsets, iNKT1, iNKT2, and iNKT17. Here, we show that T cell antigen receptor (TCR) signal strength governs the development of iNKT
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A characteristic feature of asthma is the aberrant accumulation, differentiation or function of memory CD4(+) T cells that produce type 2 cytokines (TH2 cells). By mapping genome-wide histone modification profiles for subsets of T cells isolated from peripheral blood of
Giuliana P Mognol et al.
Proceedings of the National Academy of Sciences of the United States of America, 114(13), E2776-E2785 (2017-03-12)
T-cell exhaustion is a progressive loss of effector function and memory potential due to persistent antigen exposure, which occurs in chronic viral infections and cancer. Here we investigate the relation between gene expression and chromatin accessibility in CD8+ tumor-infiltrating lymphocytes
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Instructions
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| SEQR-500RXN | 04061837018114 |
| SEQR-10RXN | 04061837018107 |
| SEQR-50RXN | 04061837018121 |
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