SAB4200129

Anti-Phospholipase A2 (iPLA2) antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody

• Synonyme(s) : Anti-CaI-PLA2, Anti-GVI, Anti-INAD1, Anti-IPLA2-VIA, Anti-PARK14, Anti-PLA2, Anti-PLA2G6, Anti-PNPLA9, Anti-Phospholipase A2, group VI (cytosolic, calcium-independent), Anti-iPLA2
• Numéro MDL: MFCD00803786
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Conjugué: unconjugated
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: buffered aqueous solution
• Poids mol.: ~85 kDa (and ~95 kDa human iPLA2 and ~95 kDa mouse and rat iPLA2.)
• Espèces réactives: human, mouse, rat
• Concentration: ~1.5 mg/mL
• Technique(s): western blot: 1.5-3.0 μg/mL using using extracts of HEK-293T cells overexpressing human iPLA₂, RAW264 cell lysates and rat pancreas extracts (S1 fraction).
• Numéro d'accès UniProt: O60733
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... PLA2G6(8398) ; mouse ... pla2g6(53357) ; rat ... pla2g6(360426)

Description

Description générale

Calcium-independent phospholipase A2 (iPLA2) belongs to the group six of phospholipase A2 (PLA2) enzymes. The protein is encoded by the gene mapped to human chromosome 22q13.1. iPLA2 is an intracellular enzyme. It is present both in the cytosol and in membrane fractions. The encoded protein contains 752 amino acids and has a mass of 85kDa. It is characterized with a eight ankyrin repeats and a catalytic domain.

Application

Anti-Phospholipase A2 (iPLA2) antibody produced in rabbit has been used in western blotting.

Actions biochimiques/physiologiques

Calcium-independent phospholipase A2 (iPLA2) in the absence of Ca²⁺, catalyzes the hydrolysis of the Sn-2 ester bond in phospholipids to produce free fatty acids and lysophospholipids. iPLA2 might play an essential role in activating the receptor-mediated signaling pathway. Additionally, it also yields precursors for bioactive compounds such as eicosanoid and platelet-activating factor. The encoded protein performs housekeeping role in phospholipid remodeling.

Forme physique

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable