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SAB4200025

Sigma-Aldrich

Anti-VPS4 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonyme(s) :

Anti-Vacuolar protein sorting 4

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~50 kDa

Espèces réactives

rat, human, mouse

Concentration

~1.0 mg/mL

Technique(s)

immunoprecipitation (IP): 2-4 μg using lysates of human HeLa cells
western blot: 1-2 μg/mL using whole extracts of mouse 3T3 or rat NRK cells

Numéro d'accès UniProt

Q9UN37

Application(s)

research pathology

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... VPS4A(27183)
mouse ... Vps4a(116733)
rat ... Vps4a(246772)

Description générale

Vacuolar protein sorting-associated protein 4 (Vps4) is a member of the AAA protein family (ATPases associated with diverse cellular activities). Vps4 exists as two orthologs in humans (VPS4A and VPS4B). The gene encoding Vps4A is mapped to chromosome 16q22, while the gene for Vps4B resides on chromosome 18q21-q22.

Spécificité

Anti-VPS4 recognizes human, mouse, and rat VPS4A and VPS4B.

Application

Anti-VPS4 antibody has been used in
  • immunoprecipitation
  • immunofluorescence Confocal Microscopy
  • immunohistochemistry
  • western blotting

Actions biochimiques/physiologiques

Vacuolar protein sorting-associated protein 4 (VPS4) mediates endosomal membrane protein sorting, retroviral budding and cytokinesis by recognizing membrane-associated ESCRT-III assemblies and catalyzing their disassembly.

Forme physique

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Stockage et stabilité

Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Shimon Harel et al.
Biophysical journal, 121(21), 4229-4238 (2022-09-01)
The assembly and budding of newly formed human immunodeficiency virus-1 (HIV-1) particles occur at the plasma membrane of infected cells. Although the molecular basis for viral budding has been studied extensively, investigation of its spatiotemporal characteristics has been limited by
ANCHR mediates Aurora-B-dependent abscission checkpoint control through retention of VPS4
Thoresen SB, et al.
Nature Cell Biology, 16(6), 550-560 (2014)
The AAA-ATPase VPS4 regulates extracellular secretion and lysosomal targeting of alpha-synuclein
Hasegawa T, et al.
PLoS ONE, 6(12), e29460-e29460 (2011)
Aiqin Hu et al.
BMC genetics, 20(1), 7-7 (2019-01-13)
Vacuolar protein sorting-associated protein 4B (VPS4B) is a member of the ATP enzyme AAA protein family, and is mainly involved in protein degradation and cell membrane fusion. Recently, a dominant mutation in this gene was identified in human dentin dysplasia
VPS4 is a dynamic component of the centrosome that regulates centrosome localization of $\gamma$-tubulin, centriolar satellite stability and ciliogenesis
Ott C, et al.
Scientific reports, 8(1), 3353-3353 (2018)
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