S5447

Anti-SIRT1 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonyme(s) : Anti-S. cerevisiae, HOMOLOG-LIKE 1, Anti-SIR2, Anti-SIR2-α, Anti-SIR2L1, Anti-Sirtuin 1
• Numéro MDL: MFCD08705602
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Conjugué: unconjugated
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen ~120 kDa
• Espèces réactives: human
• Concentration: ~1.0 mg/mL
• Technique(s): immunoprecipitation (IP): 5-10 μg using extracts of Jurkat cells; western blot: 2.5-5 μg/mL using whole extracts of Jurkat cells
• Numéro d'accès UniProt: Q96EB6
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... SIRT1(23411) ; mouse ... Sirt1(93759) ; rat ... Sirt1(309757)

Description

Description générale

Anti-SIRT1 recognizes human SIRT1. Sirtuin 1 (SIRT1) is encoded by the gene mapped to human chromosome 10q21.3. The encoded protein belongs to the silent information regulator (SIR) family and is a class of HDAC (histone deacetylase). SIRT1 is expressed in various tissues including brain, liver, pancreas, adipose tissue, and skeletal muscle. SIRT1 is also known as the longevity gene.

Immunogène

synthetic peptide corresponding to amino acids 580-593 of human SIRT1, conjugated to KLH via an N-terminal cysteine residue. The corresponding sequence differs by three amino acids in rat and mouse Sirt1.

Application

Anti-SIRT1 antibody produced in rabbit has been used in immunohistochemistry.

Actions biochimiques/physiologiques

Sirtuin 1 (SIRT1) functions as a regulator of various metabolic pathways, and influences the pathophysiology of several metabolic diseases. It is a regulator of protein deacetylation, and is a candidate therapeutic target in non-alcoholic fatty liver disease (NAFLD), amyotrophic lateral sclerosis (ALS), kidney disease, and pulmonary disease. It also participates in tumorigenesis, and whether it functions as an oncogene or as a tumor suppressor depends upon the tumor type. In pancreatic ductal adenocarcinoma (PDAC), its elevated expression is linked with poor prognosis, and in non-small-cell lung cancer (NSCLC), it suppresses the expression of tumor suppressor p27. The encoded protein inhibits the terminal differentiation in dividing myocytes by binding to the myoD cofactor, pCAF. It is also thought to function as a suppressor of cardiovascular disorders, such as myocardial infarction, or neurodegenerative diseases, such as Alzheimer′s disease (AD) or Parkinson′s disorder (PD).

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Stockage et stabilité

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frostfree” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Autres remarques

In order to obtain the best results using various techniques and preparations, we recommend determining the optimal working dilutions by titration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)