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R7876

Sigma-Aldrich

Ribonucleic acid, transfer from wheat germ

Type V, 15-19 units/mg solid

Synonyme(s) :

Transfer RNA, tRNA

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About This Item

Numéro CAS:
9014-25-9
Numéro MDL:
MFCD00132200
Code UNSPSC :
12352106
Nomenclature NACRES :
NA.55

Qualité

for molecular biology

Niveau de qualité

300

Type

Type V

Forme

lyophilized powder

Activité spécifique

15-19 units/mg solid

Conditions de stockage

desiccated

Solubilité

H2O: ~2 mg/mL, clear to hazy

Température de stockage

2-8°C

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Description générale

Wheat germ tRNA contains many different specific tRNA molecules.

Application

Crude transfer RNA (tRNA), purified from wheat germ, contains many specific tRNA molecules that can be separated by different processes. Experiments for detailed investigation of structure and function using in vitro translation systems often require highly purified tRNA, which can be achieved by combinations of chromatographic separations and reversible biochemical modification of the tRNA. Wheat germ tRNA has been used for the purification of RNAse Z (tRNA 3′- processing endonucleases) from wheat germ. The purification included six steps with the most efficient one using an affinity column made of wheat germ tRNA, which the RNAse Z bound tightly.
Suitable for use as a carrier in nucleic acid purification and precipitation.

Composants

tRNA is provided as a lyophilized powder that is soluble in water at a concentration of 2 mg/ml.

Définition de l'unité

One unit will yield an absorbance of 1.0 at 260 nm in 1.0 mL of water (1 cm light path).

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

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