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PROTIA

ProteoPrep® Immunoaffinity Albumin & IgG Depletion Kit

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A propos de cet article

NACRES:
NA.56
UNSPSC Code:
41106500
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technique(s)

immunodepletion: suitable

Quality Segment

storage temp.

2-8°C

General description

The ProteoPrep® Immunoaffinity medium in the prepacked spin columns is a mixture of two-beaded media containing recombinantly expressed, small single-chain antibody ligands, resulting in low non-specific binding and high capacity. The ProteoPrep Immunoaffinity Albumin and IgG Depletion Kit has been designed to specifically remove albumin and IgG from human serum (25-50μL) in preparation of samples for proteomics analysis, two-dimensional electrophoresis (2DE) or liquid chromatography (LC). This kit is also effective in depleting albumin and IgG from mouse and guinea pig serum.
ProteoPrep® Immunoaffinity Albumin and IgG Depletion Kit might be a useful tool for novel biomarker discovery.

Application

ProteoPrep® Immunoaffinity Albumin & amp; IgG Depletion Kit has been used in:
  • umbilical cord serum proteomics
  • human plasma (HP) proteomics
  • proteomic profile-based screening of potential protein biomarkers in the urine of patients with nephrotic syndrome

Features and Benefits

  • High Capacity removes more interfering abundant proteins
  • High Specifity ensures you see an accurate and more complete proteome
  • Buffer systems are compatible with 2 dimensional electrophoresis so there are no intermediate time consuming steps that might result in protein loss

Legal Information

ProteoPrep is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

For U.S. Customers: Contains mercury; Do not place in trash - dispose according to local, state, or federal laws.

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PROTTOTPROTMEMPROTDT
description

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description

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description

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description

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technique(s)

immunodepletion: suitable

technique(s)

-

technique(s)

-

technique(s)

analytical sample preparation: suitable

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

15-25°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200


signalword

Warning

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1

Classe de stockage

10 - Combustible liquids

wgk

WGK 3



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Questions

1–3 of 3 Questions  
  1. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  2. My protein samples contain urea.  Can I still use the ProteoPrep® Immunoaffinity Albumin & IgG Depletion Kit, Product PROTIA, for depletion?

    1 answer
    1. The presence of urea can reduce the depletion of albumin with PROTIA.  We would suggest a buffer exchange prior to depletion using either dialysis or 5 KDa NMWL cut-off spin filters.

      Helpful?

  3. Can I use the ProteoPrep® Immunoaffinity Albumin & IgG Depletion Kit, Product PROTIA, on samples besides serum?

    1 answer
    1. Besides human serum or plasma samples, we have not specifically tested other human biological fluids with this kit.  However, in principle, there is no reason not to try the kit with other fluid samples.

      Helpful?

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