P9172

Pyronin Y

for NA electrophoresis

• Synonyme(s) : Pyronin Y, Pyronin G, Pyronin J
• Formule empirique (notation de Hill): C₁₇H₁₉ClN₂O
• Numéro CAS: 92-32-0
• Poids moléculaire : 302.80
• Numéro C.I. (Colour Index): 45005
• Numéro Beilstein : 3795789
• Numéro CE : 202-147-8
• Numéro MDL: MFCD00011725
• Code UNSPSC : 12171500
• ID de substance PubChem : 57654569
• Nomenclature NACRES : NA.52

Propriétés

• Qualité: for molecular biology
• Forme: powder
• Composition: Dye content, ≥45%
• Technique(s): nucleic acid detection: suitable
• Pf: 250-260 °C (lit.)
• Solubilité: water: 10 mg/mL, dark red
• Adéquation: suitable for nucleic acid staining
• Température de stockage: room temp
• Chaîne SMILES: [Cl-].CN(C)c1ccc2C=C3C=C\C(C=C3Oc2c1)=[N+](/C)C
• InChI: 1S/C17H19N2O.ClH/c1-18(2)14-7-5-12-9-13-6-8-15(19(3)4)11-17(13)20-16(12)10-14;/h5-11H,1-4H3;1H/q+1;/p-1
• Clé InChI: INCIMLINXXICKS-UHFFFAOYSA-M

Description

Application

Pyronin Y has been used:

• in the purification of quiescent and cycling hematopoietic stem cells and staining of bone marrow cells (HSCs)
• as a component of the polychlorinated biphenyls (PCBS) solution for staining mammary gland side population cells (MG-SPs)
• staining of CD34⁺ cells

Actions biochimiques/physiologiques

Pyronin Y is a cationic dye that intercalates RNA and is visible as a red band during electrophoresis. It has also been shown to accumulate in mitochondria of viable cells. In conjuction with Methyl Green or Nancy-520, Pyronin Y can be used as a fluorescent differential stain of nucleic acids in paraffin tissue sections.

Notes préparatoires

This product is soluble in water 1 mg/ml, yielding a clear dark red solution.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)