P6749

Anti-p53 antibody, Mouse monoclonal

clone PAb1801, purified from hybridoma cell culture

• Synonyme(s) : Anti-TRP53, Anti-Transformation-related protein 53
• Numéro MDL: MFCD00164709
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 200
• Conjugué: unconjugated
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: PAb1801, monoclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen ~53 kDa
• Espèces réactives: human
• Concentration: ~2 mg/mL
• Technique(s): immunohistochemistry: suitable; immunoprecipitation (IP): suitable; indirect ELISA: suitable; western blot: 0.03-0.06 μg/mL using total cell extract of A431 cells
• Isotype: IgG1
• Numéro d'accès UniProt: P04637
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... TP53(7157)

Description

Description générale

Anti-p53 antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma PAb1801 produced by the fusion of mouse myeloma cells (SP2 cells) and splenocytes from BALB/c mice immunized with recombinant human wild type p53. Anti-p53 antibody, Mouse monoclonal recognizes human p53.
p53 is a tumor suppressor gene expressed in a wide variety of tissues. It is a tetrameric nuclear DNA-binding phosphoprotein. The gene encoding it is localized on human chromosome 17p13.1 and mouse chromosome 11.

Application

Monoclonal Anti-p53-Biotin antibody produced in mouse has been used for Western Blotting.

Actions biochimiques/physiologiques

Tumor suppressor p53 has the capability to induce cell cycle arrest and has a role in DNA repair, senescence and apoptosis. It binds to Simian vacuolating virus 40 (SV40) T-antigen and human papilloma virus E6 protein. The p53 gene is mutated in various cancers, such as of the breast, ovarian, bladder, colon and lung.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)