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P2850

Sigma-Aldrich

Phosphatase Inhibitor Cocktail 1

DMSO solution, mixture of inhibitors

Synonyme(s) :

Phosphatase Inhibitor

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About This Item

Numéro CE :
200-664-3
Code UNSPSC :
12352200
Nomenclature NACRES :
NA.54
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Nom du produit

Phosphatase Inhibitor Cocktail 1, DMSO solution

Forme

DMSO solution

Niveau de qualité

300

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Description générale

Phosphatase Inhibitor Cocktail 1 includes a mixture of inhibitors that will inhibit the L-isozymes of alkaline phosphatase as well as serine/threonine protein phosphatases such as PP1 and PP2A. This product contains microcystinLR, cantharidin, and (−)-p-bromotetramisole. Phosphatase Inhibitor Cocktail 1 has been replaced with P0044.

Application

Phosphatase Inhibitor Cocktail 1 has been used in nuclear protein isolation and electrophoretic mobility shift assay (EMSA) for catenin activation.[1] It has also been used in western blot analysis.[2][3]

Actions biochimiques/physiologiques

Phosphatases are active regulators of a number of important biological processes. Inhibition of phosphatases by small molecules is useful to study its biological role.[4]

Quantité

Recommended usage is a 100-fold dilution.

Remarque sur l'analyse

Tested on cell extracts from animal tissues such as bovine liver, human placenta, and rabbit muscle, as well as on extracts from A431 and Jurkat cell lines.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

188.6 °F

Point d'éclair (°C)

87 °C

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Certificats d'analyse (COA)

Lot/Batch Number
0000317617
0000317618
0000215517
056M4081V
17160104

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Class III β-tubulin expression and in vitro resistance to microtubule targeting agents.
Stengel C, et al.
British Journal of Cancer, 102(2), 316-316 (2010)
Larger therapeutic window for steroid versus VEGF-A inhibitor in inflammatory angiogenesis: surprisingly similar impact on leukocyte infiltration.
Nakao S, et al.
Investigative Ophthalmology & Visual Science, 53(7), 3296-3302 (2012)
Discovery of protein phosphatase inhibitor classes by biology-oriented synthesis.
Noren M A, et al.
Proceedings of the National Academy of Sciences of the USA, 103(28), 10606-10611 (2006)
X Zhao et al.
Mucosal immunology, 10(6), 1553-1568 (2017-04-13)
Virulent Mycobacterium tuberculosis (Mtb) triggers necrosis in host Mϕ, which is essential for successful pathogenesis in tuberculosis. Here we demonstrate that necrosis of Mtb-infected Mϕ is dependent on the action of the cytosolic Receptor Interacting Protein Kinase 3 (RIPK3) and
Aberrant Wnt/β-catenin signaling in pancreatic adenocarcinoma.
Zeng G, et al.
Neoplasia, 8(4), 279-289 (2006)
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Questions

1–6 of 6 Questions  

  1. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  2. How stable is Product P2850, Phosphatase Inhibitor Cocktail 1?  Can I freeze it?

    1 answer

    1. This reagent, as supplied and stored at 2-8°C, will be stable for four years from the date of manufacturing. Freezing is not recommended, because it may cause precipitation of the inhibitors.

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  3. Product P2850, Phosphatase Inhibitor Cocktail 1, is solid when it arrived. Is it suitable to use?

    1 answer

    1. Since this solution is supplied in DMSO, it may solidify (melting point of DMSO is 16-19 deg C).  Simply allow it to melt before use.  This will not affect the product.

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  4. What inhibitors are present in Product P2850, Phosphatase Inhibitor Cocktail 1, and in what concentrations?

    1 answer

    1. Cantharidin (Product Code C7632) inhibits protein phosphatase 2A (PP-2A).  Bromotetramisole (Product Code B8132) inhibits alkaline phosphatases. Microcystin LR (Product Code M2912) inhibits protein phosphatases 1 and 2A (PP-1 and PP-2A). The concentrations of these inhibitors are considered proprietary.

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  5. At what concentration should I use Product P2850, Phosphatase Inhibitor Cocktail 1?

    1 answer

    1. In most circumstannces, the product is used at a 100-fold dilution.  This will inhibit the phosphatases in 5 mg of protein per mL of lysate.

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  6. Is Product P2850, Phosphatase Inhibitor Cocktail 1, suitable for use on yeast?

    1 answer

    1. As far as we know, the phosphatases in mammalian cells also appear in yeast cells. Thus both cocktails, P5726 (Phosphatase cocktail II and P2850 (Phosphatase Inhibitor cocktail I), may be needed with yeast extracts.

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