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Key Documents

P0056

Sigma-Aldrich

Anti-POU5F1 (Oct4) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonyme(s) :

Anti-OCT3, Anti-OCT4, Anti-OTF3, Anti-OTF4, Anti-Octamer-binding transcription factor 3, Anti-Pou domain, Class 5, Transcription factor 1

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~43 kDa

Espèces réactives

human

Concentration

~1.0 mg/mL

Technique(s)

immunoprecipitation (IP): 5-10 μg using NT2 cell lysate
indirect immunofluorescence: 2-5 μg/mL using paraformaldhyde-fixed F9 cells
western blot: 1-2 μg/mL using NT2 cell lysate

Numéro d'accès UniProt

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... POU5F1(5460)
mouse ... Pou5f1(18999)
rat ... Pou5f1(294562)

Description générale

POU class 5 homeobox 1 (POU5F1) recognizes defined octamer DNA element by a shared, conserved DNA-binding domain, namely the POU domain.
POU class 5 homeobox 1 (POU5F1), also known as octamer-binding transcription factor-4 (OCT-4), is expressed in embryonic stem cells and germ cells. It belongs to the POU family of transcription factors. Three splice variants of the protein (OCT4A, OCT4B and OCT4B1) have been identified. The gene encoding this transcription factor is localized on human chromosome 6p21.33.

Immunogène

synthetic peptide corresponding to amino acids 19-34 of human POU5F1, conjugated to KLH. The corresponding sequence in rat is 81% homologous.

Application

Anti-POU5F1 (Oct4) antibody produced in rabbit has been used in Western Blotting.

Actions biochimiques/physiologiques

POU class 5 homeobox 1 (POU5F1) may be a tumor initiating stem-like cell (TSC)-related marker. It has a role in stem cell pluripotency and self-renewal, and also modulates differentiation. The protein has been shown to be expressed in several cancers. POU5F1 is also vital for the induced pluripotent stem cell (iPSC) process.
POU5F1 or Oct4/Oct3 (Octamer-binding transcription factor), functions as an early developmental control gene. It has been recognized as fundamental in the maintenance of pluripotency in embryonic stem cells and in primordial germ cells. It is down regulated in all differentiated somatic cell types both in vivo and in vitro. This is being accomplished through increased DNA methylation and structural changes involving the immediate upstream regulatory region. POU5F1 serves as a marker for germ cell tumors (GCTs) eliciting pluripotentiality, especially including embryonal, seminoma/ dysgerminoma/ germinoma.

Forme physique

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


Certificats d'analyse (COA)

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Expression and Prognostic Value of Oct-4 in Astrocytic Brain Tumors
Jeanette K
PLoS ONE (2016)
Distinctive expression pattern of OCT4 variants in different types of breast cancer.
Soheili S
Cancer Biomarkers : Section A of Disease Markers (2017)
OCT4: biological functions and clinical applications as a marker of germ cell neoplasia
Cheng L, et al.
The Journal of Pathology, 211(1), 1-9 (2007)
Characterization of POU5F1 (OCT4) gene and its promoter in buffalo ESC-like cells identifies multiple transcription start sites and expression of four pseudogenes
Natwar Singh
A Comprehensive Guide to the Hazardous Properties of Chemical Substances (2012)
The evolution of class V POU domain transcription factors in vertebrates and their characterisation in a marsupial
Frankenberg S, et al.
Developmental Biology, 337, 162-170 (2010)

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