NA1020
GenElute™ PCR Clean-Up Kit
sufficient for 70 purifications
Synonyme(s) :
Gen Elute, PCR Purification
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Code UNSPSC :
41106300
Nomenclature NACRES :
NA.52
Produits recommandés
Utilisation
sufficient for 70 purifications
Technique(s)
DNA purification: suitable
Température de stockage
15-25°C
Description générale
The GenElute PCR Clean-Up Kit is designed for rapid purification of single-stranded or double-stranded PCR amplification products (100 bp to 10 kb) from other components in the reaction, such as excess primers, nucleotides, DNA polymerase, oil and salts. This kit combines the advantages of silica binding with a convenient spin column format, eliminating the need for expensive resins or toxic organic compounds such as phenol and chloroform.
DNA is bound on a silica membrane within the spin column. The bound DNA is washed and the clean, concentrated DNA is eluted in the buffer of choice. Each column can purify up to 100 μL or 10 μg of PCR amplified DNA and recover up to 95% of PCR products between 100 bp and 10 kb. More than 99% of the primers and most primer-dimers (<40 bp) are removed.
Purified DNA can be used in enzymatic reactions, conventional or automated sequencing, cloning and microarray analysis.
DNA is bound on a silica membrane within the spin column. The bound DNA is washed and the clean, concentrated DNA is eluted in the buffer of choice. Each column can purify up to 100 μL or 10 μg of PCR amplified DNA and recover up to 95% of PCR products between 100 bp and 10 kb. More than 99% of the primers and most primer-dimers (<40 bp) are removed.
Purified DNA can be used in enzymatic reactions, conventional or automated sequencing, cloning and microarray analysis.
Application
GenElute™ PCR Clean-Up Kit has been used for rapid purification of single-stranded or double-stranded PCR amplification products (100bp to 10kb) from other components in the reaction.
Purified DNA can be used in enzymatic reactions, conventional or automated sequencing, cloning and microarray analysis.
Caractéristiques et avantages
- Purifies up to 100 μl or 10 μg of PCR amplified DNA in 8 minutes
- Recovers up to 95% of PCR products between 100 bp and 10 kb
- Removes over 99% of primers and other components
- Eliminates the need to remove mineral oil by organic extraction
- 40% more purification preps supplied than market leader
- Recovers up to 95% of PCR products between 100 bp and 10 kb
- Purifies up to 100 μL or 10 μg of PCR amplified DNA in 8 minutes
- Removes over 99% of primers and other components
Autres remarques
The GenElute PCR Clean-Up Kit is designed for rapid purification of single-stranded or double-stranded PCR amplification products (100 bp to 10 kb) from other components in the reactions, such as excess primers, nucleotides, DNA polymerase, oil and salts. This kit combines the advantages of silica binding with a convenient spin column format, eliminating the need for expensive resins or toxic organic compounds such as phenol and chloroform.
Principe
The GenElute PCR Clean-Up Kit combines the advantages of silica binding with a microspin format. The DNA is bound on a silica membrane within the spin column. The bound DNA is washed and the clean, concentrated DNA is eluted in the buffer of choice. Each column can purify up to 100 μL or 10 μg of PCR amplified DNA and recover up to 95% of PCR products between 100 bp and 10 kb. More than 99% of the primers and most primer-dimers (<40 bp are removed).
Informations légales
GenElute is a trademark of Sigma-Aldrich Co. LLC
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Acute Tox. 4 Oral - Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3
Organes cibles
Central nervous system
Code de la classe de stockage
8A - Combustible corrosive hazardous materials
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Faites votre choix parmi les versions les plus récentes :
Certificats d'analyse (COA)
Lot/Batch Number
Vous ne trouvez pas la bonne version ?
Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Les clients ont également consulté
Charlotte G Cole et al.
Genome biology, 9(5), R78-R78 (2008-05-15)
Although the human genome sequence was declared complete in 2004, the sequence was interrupted by 341 gaps of which 308 lay in an estimated approximately 28 Mb of euchromatin. While these gaps constitute only approximately 1% of the sequence, knowledge
Genetic analysis of uveal melanoma by array comparative genomic hybridization before and after radiotherapy
Werner Wackernagel
Special Care in Dentistry : Official Publication of the American Association of Hospital Dentists, the Academy of Dentistry for the Handicapped, and the American Society for Geriatric Dentistry, 27.6, 286-291 (2013)
Genetic identification of intestinal microsporidia species in immunocompromised patients in Tunisia.
Najla Chabchoub et al.
The American journal of tropical medicine and hygiene, 80(1), 24-27 (2009-01-15)
Stool samples from 86 immunocompromised patients (51 human immunodeficiency virus (HIV)-infected patients and 35 patients with haematologic malignancies) were systematically screened for intestinal microspordiosis by microscopic examination and polymerase chain reaction (PCR) using universal primer V1/PMP2. Nine samples (10.5%) showed
Adriana P Rebelo et al.
Nucleic acids research, 37(20), 6701-6715 (2009-09-11)
To characterize the organization of mtDNA-protein complexes (known as nucleoids) in vivo, we have probed the mtDNA surface exposure using site-specific DNA methyltransferases targeted to the mitochondria. We have observed that DNA methyltransferases have different accessibility to different sites on
Isolation of disseminated neuroblastoma cells from bone marrow aspirates for pretreatment risk assessment by array comparative genomic hybridization.
Vandewoestyne M
International Journal of Cancer. Journal International Du Cancer, 130(5), 1098-1108 (2012)
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique