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N2513

Sigma-Aldrich

Nerve Growth Factor β rat

≥97% (SDS-PAGE), recombinant, expressed in Sf21 cells, lyophilized powder, suitable for cell culture

Synonyme(s) :

Nerve Growth Factor-β from rat

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About This Item

Numéro CAS:
86923-98-0
Numéro MDL:
MFCD00164982
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.77

product name

NGF-β from rat, recombinant, expressed in Sf21 cells, lyophilized powder, suitable for cell culture

Source biologique

rat

Niveau de qualité

200

Produit recombinant

expressed in Sf21 cells

Pureté

≥97% (SDS-PAGE)

Forme

lyophilized powder

Puissance

0.15-3.00 ng/mL EC50

Conditionnement

pkg of 0.1 mg

Technique(s)

cell culture | mammalian: suitable

Impuretés

endotoxin, tested

Solubilité

water: soluble 0.1 g/L at 20 °C

Numéro d'accès UniProt

P25427

Température de stockage

−20°C

Informations sur le gène

rat ... NGF(4803)

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Description générale

NGFβ (nerve growth factor β) is a protein involved in signal transduction, and expressed by multiple tissues such as placenta, urothelium and bladder smooth muscle cells. It is a neurotrophin and belongs to the NGF family.

Application

NGF-β from rat is used for the following applications:
  • In the preparation of DMEM medium (for the cultivation and differentiation of PC12 cells)
  • Western blotting analysis
  • For the preparation of differentiation medium (it is prepared from DMEM, in which NGF-β is one of the components)
  • In cell culture and treatment (for neuronal differentiation)
  • In the preparation of stock solution

Actions biochimiques/physiologiques

NGFβ (nerve growth factor β) is essential for the differentiation and survival of neurons. It also plays a key role in the development and maintenance of nervous system. It is also involved in angiogenesis, and facilitates tumorigenesis such as, oral, breast and prostate cancers. It has potential as marker for the diagnosis of interstitial cystitis/painful bladder syndrome (IC/PBS) and overactive bladder (OAB), and might also aid in determining the therapy for the same. It might be involved in changes in placental function, as NGF signaling is influenced by antenatal depression and selective serotonin reuptake inhibitor (SSRI) treatment.

Forme physique

Lyophilized from a 0.2 μm filtered solution in PBS and 1 M NaCl containing 50 μg of bovine serum albumin per 1 μg of cytokine

Remarque sur l'analyse

The biological activity is measured in a cell proliferation assay using the factor-dependent human erythroleukemic cell line, TF-1.

Code de la classe de stockage

13 - Non Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Guangyi Zhao et al.
Molecular medicine reports, 16(4), 4678-4684 (2017-08-30)
The present study aimed to investigate the effect of exogenous nerve growth factor (NGF) pretreatment on demyelination in the spinal cord of lidocaine‑treated rats, and explored the potential neuroprotective mechanisms of NGF. A total of 36 rats were randomly assigned
Xiao-Ping Huang et al.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 89, 124-134 (2017-02-22)
The aim of this study was to explore the effect by which the combination of Astragaloside IV (AST IV) and Ginsenoside Rg1 (Rg1) resisted autophagic injury in PC12 cells induced by oxygen glucose deprivation/reoxygenation (OGD/R). We studied the nature of
A Khodorova et al.
Neuroscience, 254, 312-323 (2013-10-08)
Nerve growth factor (NGF) augments the excitability of isolated rat sensory neurons through activation of the p75 neurotrophin receptor (p75(NTR)) and its downstream sphingomyelin signaling cascade, wherein neutral sphingomyelinase(s) (nSMase), ceramide, and the atypical protein-kinase C (aPKC), protein-kinase M zeta
Zhe Gong et al.
Journal of neuroinflammation, 15(1), 242-242 (2018-08-30)
Nod-like receptor protein 3 (NLRP3) inflammasome is a crucial factor in mediating inflammatory responses after cerebral ischemia/reperfusion (I/R), but the cellular location of NLRP3 inflammasome in cerebral I/R has yet come to a conclusion, and there is still no specific
Niels Speksnijder et al.
Journal of molecular neuroscience : MN, 48(1), 209-218 (2012-05-25)
The glucocorticoid receptor (GR) and myocyte enhancer factor 2 (MEF2) are transcription factors involved in neuronal plasticity. c-JUN, a target gene of GR and MEF2, plays a role in regulating both synaptic strength and synapse number. The aim of this
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