MSANGERV

Sanger Arrayed Whole Genome Lentiviral CRISPR Library

Mouse, Virus Format

• Synonyme(s) : Arrayed CRISPR library, Sanger CRISPR library
• Code UNSPSC : 41105904

Propriétés

• Niveau de qualité: 200
• Conditionnement: pkg of 10 μL (384-well plate)
• Concentration: 1x10⁶ VP/ml (via p24 assay)
• Application(s): CRISPR
• Conditions d'expédition: dry ice
• Température de stockage: −70°C

Description

Description générale

Two leaders in genome editing, Sigma-Aldrich and the Wellcome Trust Sanger Institute, have joined forces to make the first ever arrayed lentiviral CRISPR knockout libraries. Based upon validated techniques published in the literature, the Sanger CRISPR libraries will put your lab at the forefront of the race to make the next big discovery.

Application

Functional Genomics/Screening /Target Validation

Caractéristiques et avantages

• Vector: U6-gRNA/PGK-Puro-2A-BFP (gRNA only)
• Simplify the workflow with puromycin selection
• Illuminate CRISPR-expressing cells with BFP

Additional Features

• Better, not bigger: Two optimized clones per mouse gene reduces the time, cost, and scale of screening experiments
• Ready-to-screen: Clones are arrayed in a robotics-friendly 384-well format for high throughput screening
• Collaborative: Real-time, library validation continues

For detailed information on the Sanger library, click here

Conditionnement

384 well plates

Composants

Lentivirus Transduction Particles of gRNA-only lenti-based vectors. 2 knockout clones for every human protein-coding gene. Nearly 40,000 sequence confirmed clones


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Forme physique

10μl of lentivirus at ≥10⁶ particles/ml in ~120×384 well plates

Autres remarques

This product is for R&D use only, not for drug, household, or other uses. Lentivirus manufacturing is achieved by using 2nd generation packaging plasmids on a 3rd generation lenti-based vector. Though the lentiviral transduction particles produced are replication incompetent, it is recommended that they be treated as Risk Group Level 2 (RGL-2) organisms in laboratory handling. Follow all published RGL-2 guidelines for laboratory handling and waste decontamination.

Produits recommandés

Additional libraries are available:
Human, Glycerol Format
Mouse, Glycerol Format
Human, Virus Format

Informations légales

CRISPR Use License Agreement

Lentiviral License Agreement

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable