MP0025
Venor™ GeM Mycoplasma Detection Kit, PCR-based
Synonyme(s) :
PCR mycoplasma detection kit
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About This Item
Code UNSPSC :
12352207
Nomenclature NACRES :
NA.75
Produits recommandés
Utilisation
1 kit sufficient for 25 tests
Conditionnement
pkg of 1 kit
Conditions de stockage
dry at room temperature
Technique(s)
PCR: suitable
Application(s)
detection
microbiology
Température de stockage
2-8°C
Description générale
The Venor™ GeM Mycoplasma Detection Kit utilizes the polymerase chain reaction (PCR), which was established as the method of choice for highest sensitivity in the detection of Mycoplasma and Acholeplasma contamination in cell cultures and other cell culture derived biologicals. Detection requires as little as 1–5fg of mycoplasma DNA corresponding to 2–5 mycoplasma per sample volume. The primer set is specific to the highly conserved rRNA operon, or more specifically, the 16S rRNA coding region in the mycoplasma genome. This allows for detection of all Mycoplasma, Acholeplasma and Ureaplasma species tested so far, which are usually encountered as contaminants in cell cultures. Eukaryotic and bacterial DNA are not amplified by this kit.
Application
The PCR-based Venor™ GeM Mycoplasma Detection Kit employs PCR technology for rapid and reliable detection of mycoplasma DNA in cell cultures and virus stocks.
Composants
Does not include Taq Polymerase. Optimized for use with D9307, Taq DNA Polymerase
Stockage et stabilité
The kit components are stable during shipping at ambient temperature. Upon receipt, store at 2–8 °C. After rehydration of the primer/nucleotide mix, the positive control, and the internal control, store below –20 °C and avoid repeated freezing and thawing. For repeated testing of low sample numbers, primer/nucleotide mix and controls should be aliquoted after rehydration. By following these recommendations, the kit is stable until the expiration date stated on the label.
Informations légales
Venor is a trademark of Minerva Biolabs GmbH
Composants de kit seuls
Réf. du produit
Description
- Positive Control
- Negative Control
- PCR 10X Reaction Buffer 500 mL/vial
- Primer/Nucleotide Mix 1 mL/vial
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
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