The MINI67 kit includes enough PKH67 dye for 25 samples and enough Diluent C for 10 samples, assuming a 1 mL sample size. Additionally, the MINI67 kit with PKH67 dye is designed to stain 125 samples. It is important to note that actual results may vary depending on cell type, among other factors.
MINI67
PKH67 Green Fluorescent Cell Linker Mini Kit for General Cell Membrane Labeling
Distributed for Phanos Technologies
Synonyme(s) :
Green PKH membrane mini labeling kit
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NACRES:
NA.32
UNSPSC Code:
12352207
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pkg of 1 kit
Quality Segment
manufacturer/tradename
Distributed for Phanos Technologies
storage condition
protect from light
technique(s)
flow cytometry: suitable
fluorescence
λex 490 nm; λem 502 nm (PKH67 dye)
application(s)
cell analysis
detection
detection method
fluorometric
shipped in
ambient
storage temp.
room temp
General description
PKH67 kit is for general cell membrane labeling. It has a longer aliphatic carbon tail than PKH1 and PKH2, two other green dyes previously described for in vitro and in vivo cell tracking. Based on the longer tail length, in-house studies have consistently shown reduced cell-cell transfer for PKH67 as compared to PKH2.
Slow loss of fluorescence has been observed in in vivo studies using PKH1 and PKH2. PKH67 may exhibit similar properties since this behavior appears to be characteristic of green cell linker dyes, but not red cell linker dyes. Correlation of in vitro cell membrane retention with in vivo fluorescence half life in non-dividing cells predicts an in vivo fluorescence half life of 10-12 days for PKH67. Other green cell linker dyes with similar half lives have been used to monitor in vivo lymphocyte and macrophage trafficking over periods of 1-2 months, suggesting that PKH67 will also be useful for in vivo tracking studies of moderate length.
Slow loss of fluorescence has been observed in in vivo studies using PKH1 and PKH2. PKH67 may exhibit similar properties since this behavior appears to be characteristic of green cell linker dyes, but not red cell linker dyes. Correlation of in vitro cell membrane retention with in vivo fluorescence half life in non-dividing cells predicts an in vivo fluorescence half life of 10-12 days for PKH67. Other green cell linker dyes with similar half lives have been used to monitor in vivo lymphocyte and macrophage trafficking over periods of 1-2 months, suggesting that PKH67 will also be useful for in vivo tracking studies of moderate length.
Application
PKH67 green fluorescent cell linker mini kit for general cell membrane labeling has been used to stain:
- exosomes
- pericytes[1]
- UT-7/TPO cells
This kit is for general cell membrane labeling. Slow loss of fluorescence has been observed in in vivo studies using PKH1 and PKH2. PKH67 may exhibit similar properties since this behavior appears to be characteristic of green cell linker dyes, but not red cell linker dyes.
Other Notes
For additional technical details on PKH and CellVue® Fluorescent Cell Linker Dyes including an extensive bibliography, please visit here.
Legal Information
Distributed for Phanos Technologies, Inc.
CellVue is a registered trademark of Phanos Technologies
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Cet article | |||
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| technique(s) flow cytometry: suitable | technique(s) - | technique(s) flow cytometry: suitable | technique(s) - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| packaging pkg of 1 kit | packaging pkg of 1 kit | packaging pkg of 1 kit | packaging pkg of 1 kit |
| detection method fluorometric | detection method fluorometric | detection method fluorometric | detection method fluorometric |
| storage condition protect from light | storage condition protect from light | storage condition protect from light | storage condition protect from light |
| fluorescence λex 490 nm; λem 502 nm (PKH67 dye) | fluorescence λex 490 nm; λem 502 nm (PKH67 dye) | fluorescence λex 551 nm; λem 567 nm (PKH26 dye) | fluorescence λex 551 nm; λem 567 nm (PKH26 dye) |
Composants de kit seuls
Réf. du produit
Description
- Diluent C 10 mL
- PKH67 Cell Linker in ethanol 0.1 mL
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 2
Classe de stockage
3 - Flammable liquids
flash_point_f
55.4 °F - closed cup
flash_point_c
13 °C - closed cup
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how many assays can be obtained from the PKH67 green fluorescent cell linker mini kit? Additionally, approximately how many number of cells that can be labeled using the mini kit?
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