MAK190
ATP Assay Kit
Sufficient for 100 Colorimetric or Fluorometric tests
Synonyme(s) :
Adenosine triphosphate Assay Kit
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About This Item
Produits recommandés
Méthode de détection
colorimetric
fluorometric
Maladie(s) pertinente(s)
cancer; neurological disorders
Température de stockage
−20°C
Description générale
Adenosine triphosphate (ATP) is a nucleoside triphosphate formed exclusively in the mitochondria. It is a high-energy molecule described as the energy currency in all living systems. The chemical energy contained in the phosphate bond of ATP drives most cellular processes. Genetic disorders such as Leber hereditary optic neuropathy, Leigh syndrome, neuropathy, ataxia, and retinitis pigmentosa affect the generation of ATP in the mitochondria.
Application
ATP Assay Kit has been used to quantify the ATP concentration by fluorometric detection of ATP in samples.
Adéquation
Suitable for the determination of ATP in samples such as animal tissue and cell culture samples.
Principe
The ATP Colorimetric/Fluorometric Assay Kit is a sensitive assay for determining ATP in various samples (ranging from 2–10 nmole/well for the colorimetric assay and from 20–1,000 pmole/well for the fluorometric assay). ATP concentration is determined by phosphorylating glycerol, resulting in a colorimetric (570 nm) or fluorometric (lex = 535/lem = 587 nm) product proportional to the amount of ATP present.
Autres remarques
The fluorometric assay is 10-100 times more sensitive than the colorimetric assay.
Composants de kit seuls
Réf. du produit
Description
- ATP Assay Buffer
- ATP Probe, in DMSO
- ATP Converter
- Developer Mix
- ATP Standard, 1.0 μmole
Remplacé(e)(s) par
Réf. du produit
Description
Tarif
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Resp. Sens. 1
Code de la classe de stockage
10 - Combustible liquids
Point d'éclair (°F)
188.6 °F
Point d'éclair (°C)
87 °C
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