MAK145

Fluorometric Intracellular Ros Kit

sufficient for 200 fluorometric tests (red)

• Synonyme(s) : Intracellular ROS Fluorescence Assay
• Code UNSPSC : 12161503
• Nomenclature NACRES : NA.25

Propriétés

• Utilisation: sufficient for 200 fluorometric tests (red)
• Méthode de détection: fluorometric
• Maladie(s) pertinente(s): neurological disorders; cancer
• Température de stockage: −20°C

Description

Description générale

Reactive oxygen species (ROS) are generated as a result of the reduction of oxygen during aerobic respiration and by various enzymatic systems within the cell. At physiological levels, ROS contribute to cell signaling and host defense. Increased ROS generation, above the detoxification capacity of the biological system, results in oxidative stress and cellular damage. The main damage to cells results from the ROS-induced alteration of macromolecules such as polyunsaturated fatty acids in membrane lipids, essential proteins, and DNA. ROS has been implicated in disease states, such as Alzheimer′s disease, Parkinson′s disease, cancer, and aging.

Application

Fluorometric intracellular reactive oxygen species (ROS) Kit has been used for detection of intracellular ROS in live cells.

Adéquation

This kit is suitable for the detection of intracellular ROS by fluorescence microplate reader or a fluorescent microscope.

Principe

The kit provides a sensitive, one-step fluorometric assay to detect intracellular ROS (especially superoxide and hydroxyl radicals) in live cells within 1 hour incubation. ROS react with a cell-permeable sensor, resulting in a fluorometric product (λ_ex = 520/λ_em = 605 nm) proportional to the amount of ROS present The assay can be performed in either a 96 or 384 multiwell plate format using a fluorescence microplate reader.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable