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M5650

Sigma-Aldrich

Milieu essentiel minimum d′Eagle

With non-essential amino acids, Earle′s salts and sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonyme(s) :

MEM

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About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.75

product name

Milieu essentiel minimum d′Eagle, With Earle′s salts, non-essential amino acids and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Niveau de qualité

400

Stérilité

sterile-filtered

Forme

liquid

Technique(s)

cell culture | mammalian: suitable

Impuretés

endotoxin, tested

Composants

phenol red: yes
NaHCO3: yes
L-glutamine: no
sodium pyruvate: no
HEPES: no
Earle’s salts (5% CO2): yes

Conditions d'expédition

ambient

Température de stockage

2-8°C

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Description générale

Minimum Essential Medium Eagle (MEM) is a synthetic cell culture media, developed by Harry Eagle. It has higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has enhanced the uses of this medium. This formulation has been further modified by the optional elimination of calcium to permit the growth of cells in suspension culture. MEM can be used for the cultivation of a wide variety of cells grown in monolayers.

Application

Minimum Essential Medium Eagle has been used to culture:
  • HeLa cells
  • Madin-Darby canine kidney (MDCK) cells
  • chondrosarcoma cell line

This medium may be preferred for the growth of adherent cells in the presence of reduced serum or as a base for the development of a serum-free medium.

Reconstitution

Supplement with 0.292 g/L L-glutamine.

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Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Biochimica et biophysica acta, 1863(6), 1422-1435 (2017-02-27)
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